Mechanical loading by fluid shear stress of myotube glycocalyx stimulates growth factor expression and nitric oxide production

P. Juffer, A.D. Bakker, J. Klein-Nulend, R.T. Jaspers

    Research output: Contribution to JournalArticleAcademicpeer-review

    Abstract

    Skeletal muscle fibers have the ability to increase their size in response to a mechanical overload. Finite element modeling data suggest that mechanically loaded muscles in vivo may experience not only tensile strain but also shear stress. However, whether shear stress affects biological pathways involved in muscle fiber size adaptation in response to mechanical loading is unknown. Therefore, our aim was twofold: (1) to determine whether shear stress affects growth factor expression and nitric oxide (NO) production by myotubes, and (2) to explore the mechanism by which shear stress may affect myotubes in vitro. C2C12 myotubes were subjected to a laminar pulsating fluid flow (PFF; mean shear stress 0.4, 0.7 or 1.4 Pa, 1 Hz) or subjected to uni-axial cyclic strain (CS; 15 % strain, 1 Hz) for 1 h. NO production during 1-h PFF or CS treatment was quantified using Griess reagent. The glycocalyx was degraded using hyaluronidase, and stretch-activated ion channels (SACs) were blocked using GdCl
    Original languageEnglish
    Pages (from-to)411-419
    JournalCell Biochemistry and Biophysics
    Volume69
    Issue number3
    DOIs
    Publication statusPublished - 2014

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