TY - JOUR
T1 - Mechanical stimulation of bone cells using fluid flow
AU - Huesa, C.
AU - Bakker, A.D.
PY - 2012
Y1 - 2012
N2 - This chapter describes several methods suitable for mechanically stimulating monolayers of bone cells by fluid shear stress (FSS) in vitro. Fluid flow is generated by pumping culture medium through two parallel plates, one of which contains a monolayer of cells. Methods for measuring nitric oxide production by bone cells in response to FSS are also described.
AB - This chapter describes several methods suitable for mechanically stimulating monolayers of bone cells by fluid shear stress (FSS) in vitro. Fluid flow is generated by pumping culture medium through two parallel plates, one of which contains a monolayer of cells. Methods for measuring nitric oxide production by bone cells in response to FSS are also described.
UR - https://www.scopus.com/pages/publications/84555195917
UR - https://www.scopus.com/inward/citedby.url?scp=84555195917&partnerID=8YFLogxK
U2 - 10.1007/978-1-61779-415-5_36
DO - 10.1007/978-1-61779-415-5_36
M3 - Article
SN - 1064-3745
VL - 816
SP - 573
EP - 592
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -