Metabolic flexibility of a prospective bioremediator: Desulfitobacterium hafniense Y51 challenged in chemostats

Sviatlana Marozava, Raquel Vargas-López, Ye Tian, Juliane Merl-Pham, Martin Braster, Rainer U. Meckenstock, Hauke Smidt, Wilfred F.M. Röling, Hans V. Westerhoff

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Desulfitobacterium hafniense Y51 has been widely used in investigations of perchloroethylene (PCE) biodegradation, but limited information exists on its other physiological capabilities. We investigated how D. hafniense Y51 confronts the debilitating limitations of not having enough electron donor (lactate), or electron acceptor (fumarate) during cultivation in chemostats. The residual concentrations of the substrates supplied in excess were much lower than expected. Transcriptomics, proteomics and fluxomics were integrated to investigate how this phenomenon was regulated. Through diverse regulation at both transcriptional and translational levels, strain Y51 turned to fermenting the excess lactate and disproportionating the excess fumarate under fumarate- and lactate-limiting conditions respectively. Genes and proteins related to the utilization of a variety of alternative electron donors and acceptors absent from the medium were induced, apparently involving the Wood–Ljungdahl pathway. Through this metabolic flexibility, D. hafniense Y51 may be able to switch between different metabolic capabilities under limiting conditions.

Original languageEnglish
Pages (from-to)2652-2669
Number of pages18
JournalEnvironmental Microbiology
Volume20
Issue number7
Early online date19 Jun 2018
DOIs
Publication statusPublished - Jul 2018

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Desulfitobacterium hafniense
Desulfitobacterium
Fumarates
chemostat
lactates
Lactic Acid
electrons
Electrons
electron
Tetrachloroethylene
tetrachloroethylene
proteomics
transcriptomics
biodegradation
Proteomics
substrate
protein
gene
fumarates
Proteins

Cite this

Marozava, Sviatlana ; Vargas-López, Raquel ; Tian, Ye ; Merl-Pham, Juliane ; Braster, Martin ; Meckenstock, Rainer U. ; Smidt, Hauke ; Röling, Wilfred F.M. ; Westerhoff, Hans V. / Metabolic flexibility of a prospective bioremediator : Desulfitobacterium hafniense Y51 challenged in chemostats. In: Environmental Microbiology. 2018 ; Vol. 20, No. 7. pp. 2652-2669.
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Metabolic flexibility of a prospective bioremediator : Desulfitobacterium hafniense Y51 challenged in chemostats. / Marozava, Sviatlana; Vargas-López, Raquel; Tian, Ye; Merl-Pham, Juliane; Braster, Martin; Meckenstock, Rainer U.; Smidt, Hauke; Röling, Wilfred F.M.; Westerhoff, Hans V.

In: Environmental Microbiology, Vol. 20, No. 7, 07.2018, p. 2652-2669.

Research output: Contribution to JournalArticleAcademicpeer-review

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