Metagenomic analysis of bacterial community structure and diversity of lignocellulolytic bacteria in Vietnamese native goat rumen

Thi Huyen Do*, Trong Khoa Dao, Khanh Hoang Viet Nguyen, Ngoc Giang Le, Thi Mai Phuong Nguyen, Tung Lam Le, Thu Nguyet Phung, Nico M. van Straalen, Dick Roelofs, Nam Hai Truong

*Corresponding author for this work

Research output: Contribution to JournalArticle

Abstract

Objective: In a previous study, analysis of Illumina sequenced metagenomic DNA data of bacteria in Vietnamese goats' rumen showed a high diversity of putative lignocellulolytic genes. In this study, taxonomy speculation of microbial community and lignocellulolytic bacteria population in the rumen was conducted to elucidate a role of bacterial structure for effective degradation of plant materials.

Methods: The metagenomic data had been subjected into Basic Local Alignment Search Tool (BLASTX) algorithm and the National Center for Biotechnology Information (NCBI) non-redundant (NR) sequence database. Here the BLASTX hits were further processed by the Metagenome Analyzer program (MEGAN) to statistically analyze the abundance of taxa.

Results: Microbial community in the rumen is defined by dominance of Bacteroidetes compared to Firmicutes. The ratio of Firmicutes versus Bacteroidetes was 0.36:1. An abundance of Synergistetes was uniquely identified in the goat microbiome may be formed by host genotype. With regard to bacterial lignocellulose degraders, the ratio of lignocellulolytic genes affiliated with Firmicutes compared to the genes linked to Bacteroidetes was 0.11:1, in which the genes encoding putative hemicellulases, carbohydrate esterases (CEs), polysaccharide lyases (PLs) originated from Bacteroidetes were 14-20 times higher than from Firmicutes. Firmicutes seem to possess more cellulose hydrolysis capacity showing a Firmicutes/Bacteroidetes ratio of 0.35:1. Analysis of lignocellulolytic potential degraders shows that four species belonged to Bacteroidetes phylum, while two species belonged to Firmicutes phylum harbouring at least 12 different catalytic domains for all lignocellulose pretreatment, cellulose, as well as hemicellulose saccharification.

Conclusion: Based on these findings, we speculate that increasing the members of Bacteroidetes to keep low ratio of Firmicutes versus Bacteroidetes in goat rumen has resulted most likely in an increased lignocellulose digestion.

Original languageEnglish
Pages (from-to)738-747
Number of pages10
JournalAsian-Australasian journal of animal sciences
Volume31
Issue number5
Early online date18 Sep 2017
DOIs
Publication statusPublished - May 2018

    Fingerprint

Keywords

  • Bacterial Structure
  • Illumina De novo Sequencing
  • Lignocellulolytic Bacteria
  • Vietnamese Goat

Cite this