Methanol oxidation in a spontaneous mutant of Thiosphaera pantotropha with a methanol-positive phenotype is catalysed by a dye-linked ethanol dehydrogenase

Joyce Ras; Marten J. Hazelaar; Lesley A. Robertson; J. Gijs Kuenen; Rob J M Van Spanning; Adriaan H. Stouthamer; Nellie Harms

doi:10.1016/0378-1097(95)00051-6

Methanol oxidation in a spontaneous mutant of Thiosphaera pantotropha with a methanol-positive phenotype is catalysed by a dye-linked ethanol dehydrogenase

Joyce Ras, Marten J. Hazelaar, Lesley A. Robertson, J. Gijs Kuenen, Rob J M Van Spanning, Adriaan H. Stouthamer, Nellie Harms^*

^*Corresponding author for this work

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Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

A spontaneous Thiosphaera pantotropha mutant (Tp9002) that is able to grow on methanol has been isolated. With hybridization experiments it has been demonstrated that mxaF, the gene encoding the large subunit of methanol dehydrogenase, is absent from T. pantotropha. In Tp9002, a dye-linked enzyme activity was found with a substrate specificity similar to that of the dye-linked ethanol dehydrogenase from Pseudomonas aeruginosa. The N-terminus of a 26-kDa cytochrome c, exclusively synthesized in Tp9002, is homologous to the N-terminus of the electron acceptor of ethanol dehydrogenase. These results suggest that in Tp9002 a dye-linked ethanol dehydrogenase is responsible for methanol oxidation, using a 26-kDa cytochrome c as electron acceptor.

Original language	English
Pages (from-to)	159-164
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	127
Issue number	3
DOIs	https://doi.org/10.1016/0378-1097(95)00051-6
Publication status	Published - 1 Apr 1995

Keywords

Ethanol dehydrogenase
Methanol dehydrogenase
Paracoccus denitrificans
Thiosphaera pantotropha

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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@article{88bf3ea850864680b0c2c2bea3edddbc,

title = "Methanol oxidation in a spontaneous mutant of Thiosphaera pantotropha with a methanol-positive phenotype is catalysed by a dye-linked ethanol dehydrogenase",

abstract = "A spontaneous Thiosphaera pantotropha mutant (Tp9002) that is able to grow on methanol has been isolated. With hybridization experiments it has been demonstrated that mxaF, the gene encoding the large subunit of methanol dehydrogenase, is absent from T. pantotropha. In Tp9002, a dye-linked enzyme activity was found with a substrate specificity similar to that of the dye-linked ethanol dehydrogenase from Pseudomonas aeruginosa. The N-terminus of a 26-kDa cytochrome c, exclusively synthesized in Tp9002, is homologous to the N-terminus of the electron acceptor of ethanol dehydrogenase. These results suggest that in Tp9002 a dye-linked ethanol dehydrogenase is responsible for methanol oxidation, using a 26-kDa cytochrome c as electron acceptor.",

keywords = "Ethanol dehydrogenase, Methanol dehydrogenase, Paracoccus denitrificans, Thiosphaera pantotropha",

author = "Joyce Ras and Hazelaar, {Marten J.} and Robertson, {Lesley A.} and Kuenen, {J. Gijs} and {Van Spanning}, {Rob J M} and Stouthamer, {Adriaan H.} and Nellie Harms",

year = "1995",

month = apr,

day = "1",

doi = "10.1016/0378-1097(95)00051-6",

language = "English",

volume = "127",

pages = "159--164",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Wiley-Blackwell",

number = "3",

}

Methanol oxidation in a spontaneous mutant of Thiosphaera pantotropha with a methanol-positive phenotype is catalysed by a dye-linked ethanol dehydrogenase. / Ras, Joyce; Hazelaar, Marten J.; Robertson, Lesley A. et al.
In: FEMS Microbiology Letters, Vol. 127, No. 3, 01.04.1995, p. 159-164.

Research output: Contribution to Journal › Article › Academic › peer-review

TY - JOUR

T1 - Methanol oxidation in a spontaneous mutant of Thiosphaera pantotropha with a methanol-positive phenotype is catalysed by a dye-linked ethanol dehydrogenase

AU - Ras, Joyce

AU - Hazelaar, Marten J.

AU - Robertson, Lesley A.

AU - Kuenen, J. Gijs

AU - Van Spanning, Rob J M

AU - Stouthamer, Adriaan H.

AU - Harms, Nellie

PY - 1995/4/1

Y1 - 1995/4/1

N2 - A spontaneous Thiosphaera pantotropha mutant (Tp9002) that is able to grow on methanol has been isolated. With hybridization experiments it has been demonstrated that mxaF, the gene encoding the large subunit of methanol dehydrogenase, is absent from T. pantotropha. In Tp9002, a dye-linked enzyme activity was found with a substrate specificity similar to that of the dye-linked ethanol dehydrogenase from Pseudomonas aeruginosa. The N-terminus of a 26-kDa cytochrome c, exclusively synthesized in Tp9002, is homologous to the N-terminus of the electron acceptor of ethanol dehydrogenase. These results suggest that in Tp9002 a dye-linked ethanol dehydrogenase is responsible for methanol oxidation, using a 26-kDa cytochrome c as electron acceptor.

AB - A spontaneous Thiosphaera pantotropha mutant (Tp9002) that is able to grow on methanol has been isolated. With hybridization experiments it has been demonstrated that mxaF, the gene encoding the large subunit of methanol dehydrogenase, is absent from T. pantotropha. In Tp9002, a dye-linked enzyme activity was found with a substrate specificity similar to that of the dye-linked ethanol dehydrogenase from Pseudomonas aeruginosa. The N-terminus of a 26-kDa cytochrome c, exclusively synthesized in Tp9002, is homologous to the N-terminus of the electron acceptor of ethanol dehydrogenase. These results suggest that in Tp9002 a dye-linked ethanol dehydrogenase is responsible for methanol oxidation, using a 26-kDa cytochrome c as electron acceptor.

KW - Ethanol dehydrogenase

KW - Methanol dehydrogenase

KW - Paracoccus denitrificans

KW - Thiosphaera pantotropha

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U2 - 10.1016/0378-1097(95)00051-6

DO - 10.1016/0378-1097(95)00051-6

M3 - Article

AN - SCOPUS:0028917006

SN - 0378-1097

VL - 127

SP - 159

EP - 164

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 3

ER -

Methanol oxidation in a spontaneous mutant of Thiosphaera pantotropha with a methanol-positive phenotype is catalysed by a dye-linked ethanol dehydrogenase

Abstract

Keywords

UN SDGs

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