Abstract
The rapidly growing, competitive biopharmaceutical market requires tight bioprocess monitoring. An integrated, automated platform for the routine online/at-line monitoring of key factors in the cell culture medium could greatly improve process monitoring. Mono- and disaccharides, as the main energy and carbon source, are one of these key factors. A CE-LIF method was developed for the analysis of several mono- and disaccharides, considering requirements and restrictions for analysis in an integrated, automated monitoring platform, such as the possibility for miniaturization to microchip electrophoresis. Analysis was performed after fluorescent derivatization with 8-aminopyrene-1,3,6-trisulfonic acid. The derivatisation reaction and the separation BGE were optimized using design of experiments. The developed method is applicable to the complex matrix of cell culture medium and proved transferable to microchip electrophoresis.
| Original language | English |
|---|---|
| Pages (from-to) | 922-929 |
| Number of pages | 8 |
| Journal | Electrophoresis |
| Volume | 43 |
| Issue number | 9-10 |
| Early online date | 12 Sept 2021 |
| DOIs | |
| Publication status | Published - May 2022 |
Bibliographical note
Special Issue: Electromigration and liquid chromatography techniques in pharmaceutical analysis 2022.© 2021 Kantisto BV. Electrophoresis published by Wiley-VCH GmbH.
Funding
This research was part of the iConsensus project, a Joint Undertaking of the Innovative Medicines Initiative 2 (grant agreement No 777397), which receives support from the European Union Horizon 2020research and innovation programme and EFPIA partners Sanofi, GSK, Bayer, Rentschler Biopharma, UCB, Byondis, and Pfizer. Meeri Mäkinen and Atefeh Shokri (Department of Industrial Biotechnology, KTH Royal Institute of Technology) are acknowledged for the cell culture medium samples. The authors gratefully acknowledge Byondis for use of their facilities, Agilent Technologies Netherlands for providing CE instrumentation, and Micronit B.V. for facilitating ME measurements. (). This research was part of the iConsensus project, a Joint Undertaking of the Innovative Medicines Initiative 2 grant agreement No 777397 , which receives support from the European Union Horizon 2020 research and innovation programme and EFPIA partners Sanofi, GSK, Bayer, Rentschler Biopharma, UCB, Byondis, and Pfizer. Meeri Mäkinen and Atefeh Shokri (Department of Industrial Biotechnology, KTH Royal Institute of Technology) are acknowledged for the cell culture medium samples. The authors gratefully acknowledge Byondis for use of their facilities, Agilent Technologies Netherlands for providing CE instrumentation, and Micronit B.V. for facilitating ME measurements
| Funders | Funder number |
|---|---|
| European Union Horizon 2020research | |
| Kungliga Tekniska Högskolan | |
| Bayer | |
| UCB | |
| Rentschler Biopharma | |
| GlaxoSmithKline | |
| Department of Industrial Biotechnology | |
| Sanofi | |
| Innovative Medicines Initiative | |
| Pfizer | |
| Horizon 2020 | |
| Horizon 2020 Framework Programme | 777397 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
-
SDG 7 Affordable and Clean Energy
Fingerprint
Dive into the research topics of 'Method development for mono- and disaccharides monitoring in cell culture medium by capillary and microchip electrophoresis'. Together they form a unique fingerprint.Cite this
- APA
- Author
- BIBTEX
- Harvard
- Standard
- RIS
- Vancouver