Microarray-based identification of clinically relevant vaginal bacteria in relation to bacterial vaginosis

Joke A M Dols; Pieter W Smit; Remco Kort; Gregor Reid; Frank H J Schuren; Hugo Tempelman; Tj Romke Bontekoe; Hans Korporaal; Mathilde E Boon

doi:10.1016/j.ajog.2010.11.012

Microarray-based identification of clinically relevant vaginal bacteria in relation to bacterial vaginosis

Joke A M Dols, Pieter W Smit, Remco Kort, Gregor Reid, Frank H J Schuren, Hugo Tempelman, Tj Romke Bontekoe, Hans Korporaal, Mathilde E Boon

AIMMS

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

OBJECTIVE: The objective was to examine the use of a tailor-made DNA microarray containing probes representing the vaginal microbiota to examine bacterial vaginosis.

STUDY DESIGN: One hundred one women attending a health center for HIV testing in South Africa were enrolled. Stained, liquid-based cytology slides were scored for bacterial vaginosis. An inventory of organisms was obtained using microarray technology, probing genera associated with bacterial vaginosis in more detail, namely Gardnerella, Atopobium, Dialister, Leptotrichia, Megasphaera, Mobiluncus, Peptostreptococcus, Prevotella, and Sneathia.

RESULTS: Of 101 women, 34 were diagnosed positive for bacterial vaginosis. This condition was associated with an increased microbial diversity. It is no longer useful to base the diagnosis of bacterial vaginosis on Gardnerella alone. Rather, its presence with Leptotrichia and Prevotella species, and especially Atopobium was more indicative of an aberrant state of the vaginal flora.

CONCLUSION: To understand the vaginal microbiota in more detail, microarray-based identification can be used after microscopic scoring.

Original language	English
Pages (from-to)	305.e1-7
Journal	American Journal of Obstetrics and Gynecology
Volume	204
Issue number	4
DOIs	https://doi.org/10.1016/j.ajog.2010.11.012
Publication status	Published - Apr 2011

Keywords

DNA, Bacterial
Female
Gram-Negative Bacteria
HIV Infections
Humans
Microscopy
Oligonucleotide Array Sequence Analysis
Polymerase Chain Reaction
South Africa
Vagina
Vaginosis, Bacterial
Journal Article

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ajog.2010.11.012

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title = "Microarray-based identification of clinically relevant vaginal bacteria in relation to bacterial vaginosis",

abstract = "OBJECTIVE: The objective was to examine the use of a tailor-made DNA microarray containing probes representing the vaginal microbiota to examine bacterial vaginosis.STUDY DESIGN: One hundred one women attending a health center for HIV testing in South Africa were enrolled. Stained, liquid-based cytology slides were scored for bacterial vaginosis. An inventory of organisms was obtained using microarray technology, probing genera associated with bacterial vaginosis in more detail, namely Gardnerella, Atopobium, Dialister, Leptotrichia, Megasphaera, Mobiluncus, Peptostreptococcus, Prevotella, and Sneathia.RESULTS: Of 101 women, 34 were diagnosed positive for bacterial vaginosis. This condition was associated with an increased microbial diversity. It is no longer useful to base the diagnosis of bacterial vaginosis on Gardnerella alone. Rather, its presence with Leptotrichia and Prevotella species, and especially Atopobium was more indicative of an aberrant state of the vaginal flora.CONCLUSION: To understand the vaginal microbiota in more detail, microarray-based identification can be used after microscopic scoring.",

keywords = "DNA, Bacterial, Female, Gram-Negative Bacteria, HIV Infections, Humans, Microscopy, Oligonucleotide Array Sequence Analysis, Polymerase Chain Reaction, South Africa, Vagina, Vaginosis, Bacterial, Journal Article",

author = "Dols, {Joke A M} and Smit, {Pieter W} and Remco Kort and Gregor Reid and Schuren, {Frank H J} and Hugo Tempelman and Bontekoe, {Tj Romke} and Hans Korporaal and Boon, {Mathilde E}",

year = "2011",

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doi = "10.1016/j.ajog.2010.11.012",

language = "English",

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AU - Dols, Joke A M

AU - Smit, Pieter W

AU - Kort, Remco

AU - Reid, Gregor

AU - Schuren, Frank H J

AU - Tempelman, Hugo

AU - Bontekoe, Tj Romke

AU - Korporaal, Hans

AU - Boon, Mathilde E

PY - 2011/4

Y1 - 2011/4

N2 - OBJECTIVE: The objective was to examine the use of a tailor-made DNA microarray containing probes representing the vaginal microbiota to examine bacterial vaginosis.STUDY DESIGN: One hundred one women attending a health center for HIV testing in South Africa were enrolled. Stained, liquid-based cytology slides were scored for bacterial vaginosis. An inventory of organisms was obtained using microarray technology, probing genera associated with bacterial vaginosis in more detail, namely Gardnerella, Atopobium, Dialister, Leptotrichia, Megasphaera, Mobiluncus, Peptostreptococcus, Prevotella, and Sneathia.RESULTS: Of 101 women, 34 were diagnosed positive for bacterial vaginosis. This condition was associated with an increased microbial diversity. It is no longer useful to base the diagnosis of bacterial vaginosis on Gardnerella alone. Rather, its presence with Leptotrichia and Prevotella species, and especially Atopobium was more indicative of an aberrant state of the vaginal flora.CONCLUSION: To understand the vaginal microbiota in more detail, microarray-based identification can be used after microscopic scoring.

AB - OBJECTIVE: The objective was to examine the use of a tailor-made DNA microarray containing probes representing the vaginal microbiota to examine bacterial vaginosis.STUDY DESIGN: One hundred one women attending a health center for HIV testing in South Africa were enrolled. Stained, liquid-based cytology slides were scored for bacterial vaginosis. An inventory of organisms was obtained using microarray technology, probing genera associated with bacterial vaginosis in more detail, namely Gardnerella, Atopobium, Dialister, Leptotrichia, Megasphaera, Mobiluncus, Peptostreptococcus, Prevotella, and Sneathia.RESULTS: Of 101 women, 34 were diagnosed positive for bacterial vaginosis. This condition was associated with an increased microbial diversity. It is no longer useful to base the diagnosis of bacterial vaginosis on Gardnerella alone. Rather, its presence with Leptotrichia and Prevotella species, and especially Atopobium was more indicative of an aberrant state of the vaginal flora.CONCLUSION: To understand the vaginal microbiota in more detail, microarray-based identification can be used after microscopic scoring.

KW - DNA, Bacterial

KW - Female

KW - Gram-Negative Bacteria

KW - HIV Infections

KW - Humans

KW - Microscopy

KW - Oligonucleotide Array Sequence Analysis

KW - Polymerase Chain Reaction

KW - South Africa

KW - Vagina

KW - Vaginosis, Bacterial

KW - Journal Article

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DO - 10.1016/j.ajog.2010.11.012

M3 - Article

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SP - 305.e1-7

JO - American Journal of Obstetrics and Gynecology

JF - American Journal of Obstetrics and Gynecology

IS - 4

ER -

Microarray-based identification of clinically relevant vaginal bacteria in relation to bacterial vaginosis

Abstract

Keywords

UN SDGs

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