TY - JOUR
T1 - Microarray detection and qPCR screening of potential biomarkers of Folsomia candida (Collembola: Isotomidae) exposed to Bt proteins (Cry1Ab and Cry1Ac)
AU - Yuan, Yijang
AU - Krogh, P.H.
AU - Bai, Xue
AU - Roelofs, D.
AU - Chen, Fajun
AU - Zhu-Salzman, Keyan
AU - Liang, Yuyong
AU - Sun, Yucheng
AU - Ge, Feng
PY - 2014
Y1 - 2014
N2 - The impact of Bt proteins on non-target arthropods is less understood than their effects on target organisms where the mechanism of toxic action is known. Here, we report the effects of two Bt proteins, Cry1Ab and Cry1Ac, on gene expression in the non-target collembolan, Folsomia candida. A customized microarray was used to study gene expression in F. candida specimens that were exposed to Cry1Ab and Cry1Ac. All selected transcripts were subsequently confirmed by qPCR. Eleven transcripts were finally verified, and three of them were annotated. The responses of all eleven transcripts were tested in specimens for both Cry1Ab and Cry1Ac at a series of concentrations. These transcripts were separated into two and three groups for Cry1Ab and Cry1Ac, respectively, depend on their expression levels. However, those eleven transcripts did not respond to the Bt proteins in Bt-rice residues. © 2013 Elsevier Ltd. All rights reserved.
AB - The impact of Bt proteins on non-target arthropods is less understood than their effects on target organisms where the mechanism of toxic action is known. Here, we report the effects of two Bt proteins, Cry1Ab and Cry1Ac, on gene expression in the non-target collembolan, Folsomia candida. A customized microarray was used to study gene expression in F. candida specimens that were exposed to Cry1Ab and Cry1Ac. All selected transcripts were subsequently confirmed by qPCR. Eleven transcripts were finally verified, and three of them were annotated. The responses of all eleven transcripts were tested in specimens for both Cry1Ab and Cry1Ac at a series of concentrations. These transcripts were separated into two and three groups for Cry1Ab and Cry1Ac, respectively, depend on their expression levels. However, those eleven transcripts did not respond to the Bt proteins in Bt-rice residues. © 2013 Elsevier Ltd. All rights reserved.
U2 - 10.1016/j.envpol.2013.08.014
DO - 10.1016/j.envpol.2013.08.014
M3 - Article
VL - 184
SP - 170
EP - 178
JO - Environmental Pollution
JF - Environmental Pollution
SN - 0269-7491
IS - January 2014
ER -