Microbial changes in relation to oral mucositis in autologous hematopoietic stem cell transplantation recipients

A.M.G.A. Laheij; J.E. Raber-Durlacher; R.G.A. Koppelmans; M.C.D.N.J.M. Huysmans; C. Potting; S.J.M. van Leeuwen; M.D. Hazenberg; M.T. Brennan; I. von Bültzingslöwen; J.E. Johansson; J.J. de Soet; T.M. Haverman; M.J. Buijs; B.W. Brandt; F.R. Rozema; N.M.A. Blijlevens; E. Zaura

doi:10.1038/s41598-019-53073-w

Microbial changes in relation to oral mucositis in autologous hematopoietic stem cell transplantation recipients

A.M.G.A. Laheij
, J.E. Raber-Durlacher
, R.G.A. Koppelmans
, M.C.D.N.J.M. Huysmans
, C. Potting
, S.J.M. van Leeuwen
, M.D. Hazenberg
, M.T. Brennan
, I. von Bültzingslöwen
, J.E. Johansson
, J.J. de Soet
, T.M. Haverman
, M.J. Buijs
, B.W. Brandt
, F.R. Rozema
, N.M.A. Blijlevens
, E. Zaura

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

The aim of this prospective, two center study was to investigate the dynamics of the microbial changes in relation to the development of ulcerative oral mucositis in autologous SCT (autoSCT) recipients. Fifty-one patients were diagnosed with multiple myeloma and treated with high-dose melphalan followed by autoSCT. They were evaluated before, three times weekly during hospitalization, and three months after autoSCT. At each time point an oral rinse was collected and the presence or absence of ulcerative oral mucositis (UOM) was scored (WHO scale). Oral microbiome was determined by using 16S rRNA amplicon sequencing and fungal load by qPCR. Twenty patients (39%) developed UOM. The oral microbiome changed significantly after autoSCT and returned to pre-autoSCT composition after three months. However, changes in microbial diversity and similarity were more pronounced and rapid in patients who developed UOM compared to patients who did not. Already before autoSCT, different taxa discriminated between the 2 groups, suggesting microbially-driven risk factors. Samples with high fungal load (>0.1%) had a significantly different microbial profile from samples without fungi. In conclusion, autoSCT induced significant and reversible changes in the oral microbiome, while patients who did not develop ulcerative oral mucositis had a more resilient microbial ecosystem.

Original language	English
Article number	16929
Number of pages	11
Journal	Scientific Reports
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41598-019-53073-w
Publication status	Published - 15 Nov 2019

Funding

This study was funded by the Dutch Cancer Society; Grant ACTA 2014–7468. The authors would like to thank W.A.E.J. de Wit from the Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam, University of Amsterdam and Vrije Universiteit Amsterdam and P. Eijk from the Tumor Genome Analysis Core from the Cancer Center Amsterdam, Amsterdam UMC, Vrije Universiteit Amsterdam for excellent technical support

Funders	Funder number
???publication-publication-funding-organisation-not-added???	10417
KWF Kankerbestrijding	ACTA 2014-6829
Dutch Cancer Society	ACTA 2014–7468

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 16 Peace, Justice and Strong Institutions

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https://hdl.handle.net/11245.1/572316cf-f8e0-4948-8706-db54402c09f3

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Laheij, A. M. G. A., Raber-Durlacher, J. E., Koppelmans, R. G. A., Huysmans, M. C. D. N. J. M., Potting, C., van Leeuwen, S. J. M., Hazenberg, M. D., Brennan, M. T., von Bültzingslöwen, I., Johansson, J. E., de Soet, J. J., Haverman, T. M., Buijs, M. J., Brandt, B. W., Rozema, F. R., Blijlevens, N. M. A., & Zaura, E. (2019). Microbial changes in relation to oral mucositis in autologous hematopoietic stem cell transplantation recipients. Scientific Reports, 9(1), Article 16929. https://doi.org/10.1038/s41598-019-53073-w

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title = "Microbial changes in relation to oral mucositis in autologous hematopoietic stem cell transplantation recipients",

abstract = "The aim of this prospective, two center study was to investigate the dynamics of the microbial changes in relation to the development of ulcerative oral mucositis in autologous SCT (autoSCT) recipients. Fifty-one patients were diagnosed with multiple myeloma and treated with high-dose melphalan followed by autoSCT. They were evaluated before, three times weekly during hospitalization, and three months after autoSCT. At each time point an oral rinse was collected and the presence or absence of ulcerative oral mucositis (UOM) was scored (WHO scale). Oral microbiome was determined by using 16S rRNA amplicon sequencing and fungal load by qPCR. Twenty patients (39\%) developed UOM. The oral microbiome changed significantly after autoSCT and returned to pre-autoSCT composition after three months. However, changes in microbial diversity and similarity were more pronounced and rapid in patients who developed UOM compared to patients who did not. Already before autoSCT, different taxa discriminated between the 2 groups, suggesting microbially-driven risk factors. Samples with high fungal load (>0.1\%) had a significantly different microbial profile from samples without fungi. In conclusion, autoSCT induced significant and reversible changes in the oral microbiome, while patients who did not develop ulcerative oral mucositis had a more resilient microbial ecosystem.",

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doi = "10.1038/s41598-019-53073-w",

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Laheij, AMGA, Raber-Durlacher, JE, Koppelmans, RGA, Huysmans, MCDNJM, Potting, C, van Leeuwen, SJM, Hazenberg, MD, Brennan, MT, von Bültzingslöwen, I, Johansson, JE, de Soet, JJ, Haverman, TM, Buijs, MJ , Brandt, BW, Rozema, FR, Blijlevens, NMA & Zaura, E 2019, 'Microbial changes in relation to oral mucositis in autologous hematopoietic stem cell transplantation recipients', Scientific Reports, vol. 9, no. 1, 16929. https://doi.org/10.1038/s41598-019-53073-w

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T1 - Microbial changes in relation to oral mucositis in autologous hematopoietic stem cell transplantation recipients

AU - Laheij, A.M.G.A.

AU - Raber-Durlacher, J.E.

AU - Koppelmans, R.G.A.

AU - Huysmans, M.C.D.N.J.M.

AU - Potting, C.

AU - van Leeuwen, S.J.M.

AU - Hazenberg, M.D.

AU - Brennan, M.T.

AU - von Bültzingslöwen, I.

AU - Johansson, J.E.

AU - de Soet, J.J.

AU - Haverman, T.M.

AU - Buijs, M.J.

AU - Brandt, B.W.

AU - Rozema, F.R.

AU - Blijlevens, N.M.A.

AU - Zaura, E.

PY - 2019/11/15

Y1 - 2019/11/15

N2 - The aim of this prospective, two center study was to investigate the dynamics of the microbial changes in relation to the development of ulcerative oral mucositis in autologous SCT (autoSCT) recipients. Fifty-one patients were diagnosed with multiple myeloma and treated with high-dose melphalan followed by autoSCT. They were evaluated before, three times weekly during hospitalization, and three months after autoSCT. At each time point an oral rinse was collected and the presence or absence of ulcerative oral mucositis (UOM) was scored (WHO scale). Oral microbiome was determined by using 16S rRNA amplicon sequencing and fungal load by qPCR. Twenty patients (39%) developed UOM. The oral microbiome changed significantly after autoSCT and returned to pre-autoSCT composition after three months. However, changes in microbial diversity and similarity were more pronounced and rapid in patients who developed UOM compared to patients who did not. Already before autoSCT, different taxa discriminated between the 2 groups, suggesting microbially-driven risk factors. Samples with high fungal load (>0.1%) had a significantly different microbial profile from samples without fungi. In conclusion, autoSCT induced significant and reversible changes in the oral microbiome, while patients who did not develop ulcerative oral mucositis had a more resilient microbial ecosystem.

AB - The aim of this prospective, two center study was to investigate the dynamics of the microbial changes in relation to the development of ulcerative oral mucositis in autologous SCT (autoSCT) recipients. Fifty-one patients were diagnosed with multiple myeloma and treated with high-dose melphalan followed by autoSCT. They were evaluated before, three times weekly during hospitalization, and three months after autoSCT. At each time point an oral rinse was collected and the presence or absence of ulcerative oral mucositis (UOM) was scored (WHO scale). Oral microbiome was determined by using 16S rRNA amplicon sequencing and fungal load by qPCR. Twenty patients (39%) developed UOM. The oral microbiome changed significantly after autoSCT and returned to pre-autoSCT composition after three months. However, changes in microbial diversity and similarity were more pronounced and rapid in patients who developed UOM compared to patients who did not. Already before autoSCT, different taxa discriminated between the 2 groups, suggesting microbially-driven risk factors. Samples with high fungal load (>0.1%) had a significantly different microbial profile from samples without fungi. In conclusion, autoSCT induced significant and reversible changes in the oral microbiome, while patients who did not develop ulcerative oral mucositis had a more resilient microbial ecosystem.

U2 - 10.1038/s41598-019-53073-w

DO - 10.1038/s41598-019-53073-w

M3 - Article

C2 - 31729407

SN - 2045-2322

VL - 9

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 16929

ER -

Microbial changes in relation to oral mucositis in autologous hematopoietic stem cell transplantation recipients

Abstract

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