Microbial domestication signatures of Lactococcus lactis can be reproduced by experimental evolution.

H. Bachmann, M.J. Starrenburg, D. Molenaar, M. Kleerebezem, J.E.T. van Hylckama Vlieg

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Experimental evolution is a powerful approach to unravel how selective forces shape microbial genotypes and phenotypes. To this date, the available examples focus on the adaptation to conditions specific to the laboratory. The lactic acid bacterium Lactococcus lactis naturally occurs on plants and in dairy environments, and it is proposed that dairy strains originate from the plant niche. Here we investigate the adaptation of a L. lactis strain isolated from a plant to a dairy niche by propagating it for 1000 generations in milk. Two out of three independently evolved strains displayed significantly increased acidification rates and biomass yields in milk. Genome resequencing, revealed six, seven, and 28 mutations in the three strains, including point mutations in loci related to amino acid biosynthesis and transport and in the gene encoding MutL, which is involved in DNA mismatch repair. Two strains lost a conjugative transposon containing genes important in the plant niche but dispensable in milk. A plasmid carrying an extracellular protease was introduced by transformation. Although improving growth rate and growth yield significantly, the plasmid was rapidly lost. Comparative transcriptome and phenotypic analyses confirmed that major physiological changes associated with improved growth in milk relate to nitrogen metabolism and the loss or down-regulation of several pathways involved in the utilization of complex plant polymers. Reproducing the transition from the plant to the dairy niche through experimental evolution revealed several genome, transcriptome, and phenotype signatures that resemble those seen in strains isolated from either niche. © 2012 by Cold Spring Harbor Laboratory Press.
Original languageEnglish
Pages (from-to)115-124
JournalGenome Research
Issue number22
DOIs
Publication statusPublished - 2012

Fingerprint

Lactococcus lactis
Milk
Plasmids
Growth
Genome
Phenotype
DNA Mismatch Repair
Gene Expression Profiling
Transcriptome
Point Mutation
Biomass
Genes
Domestication
Lactic Acid
Polymers
Peptide Hydrolases
Nitrogen
Down-Regulation
Genotype
Bacteria

Cite this

Bachmann, H. ; Starrenburg, M.J. ; Molenaar, D. ; Kleerebezem, M. ; van Hylckama Vlieg, J.E.T. / Microbial domestication signatures of Lactococcus lactis can be reproduced by experimental evolution. In: Genome Research. 2012 ; No. 22. pp. 115-124.
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abstract = "Experimental evolution is a powerful approach to unravel how selective forces shape microbial genotypes and phenotypes. To this date, the available examples focus on the adaptation to conditions specific to the laboratory. The lactic acid bacterium Lactococcus lactis naturally occurs on plants and in dairy environments, and it is proposed that dairy strains originate from the plant niche. Here we investigate the adaptation of a L. lactis strain isolated from a plant to a dairy niche by propagating it for 1000 generations in milk. Two out of three independently evolved strains displayed significantly increased acidification rates and biomass yields in milk. Genome resequencing, revealed six, seven, and 28 mutations in the three strains, including point mutations in loci related to amino acid biosynthesis and transport and in the gene encoding MutL, which is involved in DNA mismatch repair. Two strains lost a conjugative transposon containing genes important in the plant niche but dispensable in milk. A plasmid carrying an extracellular protease was introduced by transformation. Although improving growth rate and growth yield significantly, the plasmid was rapidly lost. Comparative transcriptome and phenotypic analyses confirmed that major physiological changes associated with improved growth in milk relate to nitrogen metabolism and the loss or down-regulation of several pathways involved in the utilization of complex plant polymers. Reproducing the transition from the plant to the dairy niche through experimental evolution revealed several genome, transcriptome, and phenotype signatures that resemble those seen in strains isolated from either niche. {\circledC} 2012 by Cold Spring Harbor Laboratory Press.",
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Microbial domestication signatures of Lactococcus lactis can be reproduced by experimental evolution. / Bachmann, H.; Starrenburg, M.J.; Molenaar, D.; Kleerebezem, M.; van Hylckama Vlieg, J.E.T.

In: Genome Research, No. 22, 2012, p. 115-124.

Research output: Contribution to JournalArticleAcademicpeer-review

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