Microsatellite loci in the soil-dwelling collembolan, Orchesella cincta.

A.W.G. van der Wurff; R. Gols; G. Ernsting; N.M. van Straalen

doi:10.1046/j.1471-8278.2001.00069.x

Microsatellite loci in the soil-dwelling collembolan, Orchesella cincta.

A.W.G. van der Wurff, R. Gols, G. Ernsting, N.M. van Straalen

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Three total genomic libraries of the springtail Orchesella cincta (Insecta; Collembola) were screened for the presence of microsatellites using phosphor and colour detection. PCR primers were successfully constructed for six dinucleotide, one trinucleotide, and one interrupted dinucleotide microsatellite repeat. None of the microsatellite arrays were longer than 11 repeat units. Individuals from three forests were investigated: five out of eight microsatellite markers were polymophic in all forests (2-5 alleles per locus) and the observed heterozygosity was 0.1-0.9 per locus.

Original language	English
Pages (from-to)	182-184
Journal	Molecular Ecology Notes
Volume	1
DOIs	https://doi.org/10.1046/j.1471-8278.2001.00069.x
Publication status	Published - 2001

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title = "Microsatellite loci in the soil-dwelling collembolan, Orchesella cincta.",

abstract = "Three total genomic libraries of the springtail Orchesella cincta (Insecta; Collembola) were screened for the presence of microsatellites using phosphor and colour detection. PCR primers were successfully constructed for six dinucleotide, one trinucleotide, and one interrupted dinucleotide microsatellite repeat. None of the microsatellite arrays were longer than 11 repeat units. Individuals from three forests were investigated: five out of eight microsatellite markers were polymophic in all forests (2-5 alleles per locus) and the observed heterozygosity was 0.1-0.9 per locus.",

author = "{van der Wurff}, A.W.G. and R. Gols and G. Ernsting and {van Straalen}, N.M.",

year = "2001",

doi = "10.1046/j.1471-8278.2001.00069.x",

language = "English",

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pages = "182--184",

journal = "Molecular Ecology Notes",

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AU - van der Wurff, A.W.G.

AU - Gols, R.

AU - Ernsting, G.

AU - van Straalen, N.M.

PY - 2001

Y1 - 2001

N2 - Three total genomic libraries of the springtail Orchesella cincta (Insecta; Collembola) were screened for the presence of microsatellites using phosphor and colour detection. PCR primers were successfully constructed for six dinucleotide, one trinucleotide, and one interrupted dinucleotide microsatellite repeat. None of the microsatellite arrays were longer than 11 repeat units. Individuals from three forests were investigated: five out of eight microsatellite markers were polymophic in all forests (2-5 alleles per locus) and the observed heterozygosity was 0.1-0.9 per locus.

AB - Three total genomic libraries of the springtail Orchesella cincta (Insecta; Collembola) were screened for the presence of microsatellites using phosphor and colour detection. PCR primers were successfully constructed for six dinucleotide, one trinucleotide, and one interrupted dinucleotide microsatellite repeat. None of the microsatellite arrays were longer than 11 repeat units. Individuals from three forests were investigated: five out of eight microsatellite markers were polymophic in all forests (2-5 alleles per locus) and the observed heterozygosity was 0.1-0.9 per locus.

U2 - 10.1046/j.1471-8278.2001.00069.x

DO - 10.1046/j.1471-8278.2001.00069.x

M3 - Article

SN - 1471-8278

VL - 1

SP - 182

EP - 184

JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

ER -

Microsatellite loci in the soil-dwelling collembolan, Orchesella cincta.

Abstract

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