Miniaturization of a transthyretin binding assay using a fluorescent probe for high throughput screening of thyroid hormone disruption in environmental samples

X. Ouyang, Jean Froment, P.E.G. Leonards, Guttorm Christensen, Knut-Erik Tollefsen, J. de Boer, Kevin V Thomas, M.H. Lamoree

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Thyroid hormone (TH) disrupting compounds are potentially important environmental contaminants due to their possible adverse neurological and developmental effects on both humans and wildlife. Currently, the most successful bio-analytical method to detect and evaluate TH disruptors, which target the plasma transport of TH in environmental samples, is the radio-ligand thyroxine-transthyretin (T4-TTR) binding assay. Yet, costly materials and tedious handling procedures prevent the use of this assay in high throughput analysis that is nowadays urgently demanded in environmental quality assessment. For the first time a miniaturized fluorescence T4-TTR binding assay was developed in a 96 well microplate and tested with eight TH disrupting compounds. For most of the compounds, the sensitivity of the newly developed assay was slightly lower than the radio-ligand binding assay, however, throughput was enhanced at least 100-fold, while using much cheaper materials. The TH disrupting potency of 22 herring gull (Larus argentatus) egg extracts, collected from two different locations (Musvær and Reiaren) in Norway, was evaluated to demonstrate the applicability of the assay for environmental samples.
Original languageEnglish
Pages (from-to)722-728
JournalChemosphere
Volume171
DOIs
Publication statusPublished - 2017

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Miniaturization
Prealbumin
Thyroid Hormones
Fluorescent Dyes
hormone
Assays
Screening
probe
Throughput
assay
Radio
ligand
Charadriiformes
Ligands
radio
Norway
Thyroxine
microplate
Ovum
environmental quality

Cite this

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title = "Miniaturization of a transthyretin binding assay using a fluorescent probe for high throughput screening of thyroid hormone disruption in environmental samples",
abstract = "Thyroid hormone (TH) disrupting compounds are potentially important environmental contaminants due to their possible adverse neurological and developmental effects on both humans and wildlife. Currently, the most successful bio-analytical method to detect and evaluate TH disruptors, which target the plasma transport of TH in environmental samples, is the radio-ligand thyroxine-transthyretin (T4-TTR) binding assay. Yet, costly materials and tedious handling procedures prevent the use of this assay in high throughput analysis that is nowadays urgently demanded in environmental quality assessment. For the first time a miniaturized fluorescence T4-TTR binding assay was developed in a 96 well microplate and tested with eight TH disrupting compounds. For most of the compounds, the sensitivity of the newly developed assay was slightly lower than the radio-ligand binding assay, however, throughput was enhanced at least 100-fold, while using much cheaper materials. The TH disrupting potency of 22 herring gull (Larus argentatus) egg extracts, collected from two different locations (Musv{\ae}r and Reiaren) in Norway, was evaluated to demonstrate the applicability of the assay for environmental samples.",
author = "X. Ouyang and Jean Froment and P.E.G. Leonards and Guttorm Christensen and Knut-Erik Tollefsen and {de Boer}, J. and Thomas, {Kevin V} and M.H. Lamoree",
year = "2017",
doi = "10.1016/j.chemosphere.2016.12.119",
language = "English",
volume = "171",
pages = "722--728",
journal = "Chemosphere",
issn = "0045-6535",
publisher = "Elsevier Limited",

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Miniaturization of a transthyretin binding assay using a fluorescent probe for high throughput screening of thyroid hormone disruption in environmental samples. / Ouyang, X.; Froment, Jean; Leonards, P.E.G.; Christensen, Guttorm; Tollefsen, Knut-Erik ; de Boer, J.; Thomas, Kevin V; Lamoree, M.H.

In: Chemosphere, Vol. 171, 2017, p. 722-728.

Research output: Contribution to JournalArticleAcademicpeer-review

TY - JOUR

T1 - Miniaturization of a transthyretin binding assay using a fluorescent probe for high throughput screening of thyroid hormone disruption in environmental samples

AU - Ouyang, X.

AU - Froment, Jean

AU - Leonards, P.E.G.

AU - Christensen, Guttorm

AU - Tollefsen, Knut-Erik

AU - de Boer, J.

AU - Thomas, Kevin V

AU - Lamoree, M.H.

PY - 2017

Y1 - 2017

N2 - Thyroid hormone (TH) disrupting compounds are potentially important environmental contaminants due to their possible adverse neurological and developmental effects on both humans and wildlife. Currently, the most successful bio-analytical method to detect and evaluate TH disruptors, which target the plasma transport of TH in environmental samples, is the radio-ligand thyroxine-transthyretin (T4-TTR) binding assay. Yet, costly materials and tedious handling procedures prevent the use of this assay in high throughput analysis that is nowadays urgently demanded in environmental quality assessment. For the first time a miniaturized fluorescence T4-TTR binding assay was developed in a 96 well microplate and tested with eight TH disrupting compounds. For most of the compounds, the sensitivity of the newly developed assay was slightly lower than the radio-ligand binding assay, however, throughput was enhanced at least 100-fold, while using much cheaper materials. The TH disrupting potency of 22 herring gull (Larus argentatus) egg extracts, collected from two different locations (Musvær and Reiaren) in Norway, was evaluated to demonstrate the applicability of the assay for environmental samples.

AB - Thyroid hormone (TH) disrupting compounds are potentially important environmental contaminants due to their possible adverse neurological and developmental effects on both humans and wildlife. Currently, the most successful bio-analytical method to detect and evaluate TH disruptors, which target the plasma transport of TH in environmental samples, is the radio-ligand thyroxine-transthyretin (T4-TTR) binding assay. Yet, costly materials and tedious handling procedures prevent the use of this assay in high throughput analysis that is nowadays urgently demanded in environmental quality assessment. For the first time a miniaturized fluorescence T4-TTR binding assay was developed in a 96 well microplate and tested with eight TH disrupting compounds. For most of the compounds, the sensitivity of the newly developed assay was slightly lower than the radio-ligand binding assay, however, throughput was enhanced at least 100-fold, while using much cheaper materials. The TH disrupting potency of 22 herring gull (Larus argentatus) egg extracts, collected from two different locations (Musvær and Reiaren) in Norway, was evaluated to demonstrate the applicability of the assay for environmental samples.

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