The response of glycolysis to exposure of glucose in non-growing S. cerevisiae cells from diauxic shift was monitored. The result was compared to a kinetic model of glycolysis with branches to glycogen, trehalose, glycerol, and succinate. Experimental data at steady-state concentrations of metabolites in the upper part agreed well between the model and experiments. Larger discrepancies were found in the lower part of glycolysis and as concerns the rates of glycerol and ethanol formation. Further improvements of the model are needed and new branches of glycolysis have to be identified and included. The control of the ethanol flux were exerted by the uptake since the flux control coefficient for glucose transport was 1.09.