Molecular diagnosis of Strongyloides stercoralis in faecal samples using real-time PCR

Jaco J. Verweij*, Marco Canales, Katja Polman, Juventus Ziem, Eric A T Brienen, Anton M. Polderman, Lisette van Lieshout

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

A real-time PCR method targeting the small subunit of the rRNA gene was developed for the detection of Strongyloides stercoralis DNA in faecal samples, including an internal control to detect inhibition of the amplification process. The assay was performed on a range of well-defined control samples (n = 145), known positive faecal samples (n = 38) and faecal samples from a region in northern Ghana where S. stercoralis infections are highly endemic (n = 212), and achieved 100% specificity and high sensitivity. The use of this assay could facilitate monitoring the prevalence and intensity of S. stercoralis infections during helminth intervention programs. Moreover, the use of this assay in diagnostic laboratories could make the introduction of molecular diagnostics feasible in the routine diagnosis of S. stercoralis infections, with a two-fold increase in the detection rate as compared with the commonly used Baermann sedimentation method.

Original languageEnglish
Pages (from-to)342-346
Number of pages5
JournalRoyal Society of Tropical Medicine and Hygiene. Transactions
Volume103
Issue number4
DOIs
Publication statusPublished - Apr 2009
Externally publishedYes

Keywords

  • Baermann sedimentation
  • Diagnosis
  • Parasitic intestinal disease
  • Real-time PCR
  • Strongyloides stercoralis
  • Strongyloidiasis

Fingerprint

Dive into the research topics of 'Molecular diagnosis of Strongyloides stercoralis in faecal samples using real-time PCR'. Together they form a unique fingerprint.

Cite this