Molecular diversity and distribution of aromatic hydrocarbon-degrading anaerobes across a landfill leachate plume.

M. Staats, M. Braster, W.F.M. Roling

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Natural attenuation of the mono-aromates benzene, toluene, ethylbenzene and xylene occurs under iron-reducing conditions in a leachate-contaminated aquifer near the Banisveld landfill, the Netherlands. The diversity of mono-aromate-degrading microorganisms was studied by targeting functional genes encoding benzylsuccinate synthase α-subunit (bssA) and 6-oxocyclohex-1-ene-1-carbonyl-CoA hydrolase (bamA). Sixty-four bssA and 188 bamA variants were sequenced from groundwater sampled along the pollution plume in 1999 and 2004. Species containing bssA sequences closest affiliated (>91%) with the betaprotebacterium Georgfuchsia toluolica were the dominant alkylbenzene degraders (89% of bssA sequences). bssA genes were found at more than 10-fold lower copy numbers than bamA genes, of which only a small fraction (<2%) was closely related to the genes of Georgfuchsia. bamA gene diversity was high and bamA-based community composition was primarily affected by dissolved organic carbon (DOC) and ferrous iron concentrations. bamA sequences closest related to Geobacteraceae were dominantly (43.2%) observed and the presence of Geobacteraceae-related bamA sequences was associated with DOC. Our results indicate a key role for specialized Georgfuchsia spp. in the degradation of alkylbenzenes, whereas Geobacteraceae are involved in degradation of aromatics other than toluene and xylene. © 2011 Society for Applied Microbiology and Blackwell Publishing Ltd.
Original languageEnglish
Pages (from-to)1216-1227
JournalEnvironmental Microbiology
Volume13
DOIs
Publication statusPublished - 2011

Fingerprint

Dive into the research topics of 'Molecular diversity and distribution of aromatic hydrocarbon-degrading anaerobes across a landfill leachate plume.'. Together they form a unique fingerprint.

Cite this