Monitoring Receptor Oligomerization by Line-Scan Fluorescence Cross-Correlation Spectroscopy

Mark A. Hink*, Marten Postma

*Corresponding author for this work

Research output: Chapter in Book / Report / Conference proceedingChapterAcademicpeer-review

Abstract

Membrane-localized receptor proteins are involved in many signaling cascades, and diffusion and oligomerization are key processes controlling their activity. In order to study these processes in living cells, fluorescence fluctuation spectroscopy techniques have been developed that allow the quantification of concentration levels, diffusion rates, and interactions between fluorescently labeled receptor proteins at the nanomolar concentration level. This chapter presents a brief introduction to the technique and a protocol to measure and quantify the diffusion and oligomerization of human histamine1 receptor complexes in living HeLa cells using line-scanning fluorescence cross-correlation spectroscopy.

Original languageEnglish
Title of host publicationMethods in Cell Biology
PublisherAcademic Press Inc.
Pages197-212
Number of pages16
DOIs
Publication statusPublished - 1 Jan 2013
Externally publishedYes

Publication series

NameMethods in Cell Biology
Volume117
ISSN (Print)0091-679X

Keywords

  • Dimerization
  • Fluorescence cross-correlation spectroscopy
  • Human histamine1 receptor
  • Line-scan FCCS

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