TY - JOUR
T1 - Mouse strain-specific responses along the gut-brain axis upon fecal microbiota transplantation from children with autism
AU - Prince, Naika
AU - Peralta Marzal, Lucia N.
AU - Roussin, Léa
AU - Monnoye, Magali
AU - Philippe, Catherine
AU - Maximin, Elise
AU - Ahmed, Sabbir
AU - Salenius, Karoliina
AU - Lin, Jake
AU - Autio, Reija
AU - Adolfs, Youri
AU - Pasterkamp, R. Jeroen
AU - Garssen, Johan
AU - Naudon, Laurent
AU - Rabot, Sylvie
AU - Kraneveld, Aletta D.
AU - Perez-Pardo, Paula
AU - On behalf of the GEMMA Consortium
N1 - Publisher Copyright:
© 2025 The Author(s). Published with license by Taylor & Francis Group, LLC.
PY - 2025
Y1 - 2025
N2 - Several factors are linked to the pathophysiology of autism spectrum disorders (ASD); however, the molecular mechanisms of the condition remain unknown. As intestinal problems and gut microbiota dysbiosis are associated with ASD development and severity, recent studies have focused on elucidating the microbiota-gut-brain axis’ involvement. This study aims to explore mechanisms through which gut microbiota might influence ASD. Briefly, we depleted the microbiota of conventional male BALB/cAnNCrl (Balb/c) and C57BL/6J (BL/6) mice prior to human fecal microbiota transplantation (hFMT) with samples from children with ASD or their neurotypical siblings. We found mouse strain-specific responses to ASD hFMT. Notably, Balb/c mice exhibit decreased exploratory and social behavior, and show evidence of intestinal, systemic, and central inflammation accompanied with metabolic shifts. BL/6 mice show less changes after hFMT. Our results reveal that gut microbiota alone induce changes in ASD-like behavior, and highlight the importance of mouse strain selection when investigating multifactorial conditions like ASD.
AB - Several factors are linked to the pathophysiology of autism spectrum disorders (ASD); however, the molecular mechanisms of the condition remain unknown. As intestinal problems and gut microbiota dysbiosis are associated with ASD development and severity, recent studies have focused on elucidating the microbiota-gut-brain axis’ involvement. This study aims to explore mechanisms through which gut microbiota might influence ASD. Briefly, we depleted the microbiota of conventional male BALB/cAnNCrl (Balb/c) and C57BL/6J (BL/6) mice prior to human fecal microbiota transplantation (hFMT) with samples from children with ASD or their neurotypical siblings. We found mouse strain-specific responses to ASD hFMT. Notably, Balb/c mice exhibit decreased exploratory and social behavior, and show evidence of intestinal, systemic, and central inflammation accompanied with metabolic shifts. BL/6 mice show less changes after hFMT. Our results reveal that gut microbiota alone induce changes in ASD-like behavior, and highlight the importance of mouse strain selection when investigating multifactorial conditions like ASD.
KW - autism spectrum disorders
KW - Fecal microbiota transplantation
KW - gut-brain axis
KW - humanized mouse model
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U2 - 10.1080/19490976.2024.2447822
DO - 10.1080/19490976.2024.2447822
M3 - Article
AN - SCOPUS:85214457331
SN - 1949-0976
VL - 17
SP - 1
EP - 25
JO - Gut microbes
JF - Gut microbes
IS - 1
M1 - 2447822
ER -