MreB-Dependent Organization of the E-coli Cytoplasmic Membrane Controls Membrane Protein Diffusion

F. Oswald, A. Varadarajan, H. Lill, E.J.G. Peterman, Y.J.M. Bollen

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

The functional organization of prokaryotic cell membranes, which is essential for many cellular processes, has been challenging to analyze due to the small size and nonflat geometry of bacterial cells. Here, we use single-molecule fluorescence microscopy and three-dimensional quantitative analyses in live Escherichia coli to demonstrate that its cytoplasmic membrane contains microdomains with distinct physical properties. We show that the stability of these microdomains depends on the integrity of the MreB cytoskeletal network underneath the membrane. We explore how the interplay between cytoskeleton and membrane affects trans-membrane protein (TMP) diffusion and reveal that the mobility of the TMPs tested is subdiffusive, most likely caused by confinement of TMP mobility by the submembranous MreB network. Our findings demonstrate that the dynamic architecture of prokaryotic cell membranes is controlled by the MreB cytoskeleton and regulates the mobility of TMPs.
Original languageEnglish
Pages (from-to)1139-1149
JournalBiophysical Journal
Volume110
Issue number5
DOIs
Publication statusPublished - 2016

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Prokaryotic Cells
Thymidine Monophosphate
Membrane Proteins
Cell Membrane
Escherichia coli
Cytoskeleton
Membrane Microdomains
Membranes
Fluorescence Microscopy
Single Molecule Imaging

Cite this

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title = "MreB-Dependent Organization of the E-coli Cytoplasmic Membrane Controls Membrane Protein Diffusion",
abstract = "The functional organization of prokaryotic cell membranes, which is essential for many cellular processes, has been challenging to analyze due to the small size and nonflat geometry of bacterial cells. Here, we use single-molecule fluorescence microscopy and three-dimensional quantitative analyses in live Escherichia coli to demonstrate that its cytoplasmic membrane contains microdomains with distinct physical properties. We show that the stability of these microdomains depends on the integrity of the MreB cytoskeletal network underneath the membrane. We explore how the interplay between cytoskeleton and membrane affects trans-membrane protein (TMP) diffusion and reveal that the mobility of the TMPs tested is subdiffusive, most likely caused by confinement of TMP mobility by the submembranous MreB network. Our findings demonstrate that the dynamic architecture of prokaryotic cell membranes is controlled by the MreB cytoskeleton and regulates the mobility of TMPs.",
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MreB-Dependent Organization of the E-coli Cytoplasmic Membrane Controls Membrane Protein Diffusion. / Oswald, F.; Varadarajan, A.; Lill, H.; Peterman, E.J.G.; Bollen, Y.J.M.

In: Biophysical Journal, Vol. 110, No. 5, 2016, p. 1139-1149.

Research output: Contribution to JournalArticleAcademicpeer-review

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AU - Oswald, F.

AU - Varadarajan, A.

AU - Lill, H.

AU - Peterman, E.J.G.

AU - Bollen, Y.J.M.

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AB - The functional organization of prokaryotic cell membranes, which is essential for many cellular processes, has been challenging to analyze due to the small size and nonflat geometry of bacterial cells. Here, we use single-molecule fluorescence microscopy and three-dimensional quantitative analyses in live Escherichia coli to demonstrate that its cytoplasmic membrane contains microdomains with distinct physical properties. We show that the stability of these microdomains depends on the integrity of the MreB cytoskeletal network underneath the membrane. We explore how the interplay between cytoskeleton and membrane affects trans-membrane protein (TMP) diffusion and reveal that the mobility of the TMPs tested is subdiffusive, most likely caused by confinement of TMP mobility by the submembranous MreB network. Our findings demonstrate that the dynamic architecture of prokaryotic cell membranes is controlled by the MreB cytoskeleton and regulates the mobility of TMPs.

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