MScarlet: A bright monomeric red fluorescent protein for cellular imaging

Daphne S. Bindels, Lindsay Haarbosch, Laura Van Weeren, Marten Postma, Katrin E. Wiese, Marieke Mastop, Sylvain Aumonier, Guillaume Gotthard, Antoine Royant, Mark A. Hink, Theodorus W.J. Gadella*

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

We report the engineering of mScarlet, a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns). We developed mScarlet starting with a consensus synthetic template and using improved spectroscopic screening techniques; mScarlet's crystal structure reveals a planar and rigidified chromophore. mScarlet outperforms existing red fluorescent proteins as a fusion tag, and it is especially useful as a Förster resonance energy transfer (FRET) acceptor in ratiometric imaging.

Original languageEnglish
Pages (from-to)53-56
Number of pages4
JournalNature Methods
Volume14
Issue number1
DOIs
Publication statusPublished - 29 Dec 2016
Externally publishedYes

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