Multimodal fluorescence imaging spectroscopy

Martijn H W Stopel, Christian Blum, Vinod Subramaniam

    Research output: Contribution to JournalArticleAcademicpeer-review

    Abstract

    Multimodal fluorescence imaging is a versatile method that has a wide application range from biological studies to materials science. Typical observables in multimodal fluorescence imaging are intensity, lifetime, excitation, and emission spectra which are recorded at chosen locations at the sample. This chapter describes how to build instrumentation that allows for multimodal fluorescence imaging and explains data analysis procedures for the observables.

    Original languageEnglish
    Pages (from-to)521-36
    Number of pages16
    JournalMethods in Molecular Biology
    Volume1076
    DOIs
    Publication statusPublished - 2014

    Keywords

    • Absorptiometry, Photon
    • Fluorescence
    • Light
    • Optical Imaging
    • Plant Cells
    • Spectrometry, Fluorescence
    • Journal Article

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