Munc18-1 expression levels control synapse recovery by regulating readily releasable pool size

R.F.G. Toonen, K. Wierda, M.S. Sons, H. de Wit, L.N. Cornelisse, A. Brussaard, J.J. Plomp, M. Verhage

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Prompt recovery after intense activity is an essential feature of most mammalian synapses. Here we show that synapses with reduced expression of the presynaptic gene munc18-1 suffer from increased depression during intense stimulation at glutamatergic, GABAergic, and neuromuscular synapses. Conversely, munc18-1 overexpression makes these synapses recover faster. Concomitant changes in the readily releasable vesicle pool and its refill kinetics were found. The number of vesicles docked at the active zone and the total number of vesicles per terminal correlated with both munc18-1 expression levels and the size of the releasable vesicle pool. These data show that varying expression of a single gene controls synaptic recovery by modulating the number of docked, release-ready vesicles and thereby replenishment of the secretion capacity. © 2006 by The National Academy of Sciences of the USA.
Original languageEnglish
Pages (from-to)18332-18337
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number48
DOIs
Publication statusPublished - 2006

Fingerprint

Dive into the research topics of 'Munc18-1 expression levels control synapse recovery by regulating readily releasable pool size'. Together they form a unique fingerprint.

Cite this