Mutagenesis of the gene encoding amicyanin of Paracoccus denitrificans and the resultant effect on methylamine oxidation

R J van Spanning, C W Wansell, W N Reijnders, L F Oltmann, A.H. Stouthamer

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

The gene encoding the blue-copper protein amicyanin was isolated from a genomic bank of Paracoccus denitrificans by using a synthetic oligonucleotide. It is located directly downstream of the gene encoding the small subunit of methylamine dehydrogenase. Amicyanin is transcribed as a precursor protein with a signal sequence, typical for periplasmic proteins. Specific inactivation of amicyanin by means of gene replacement techniques resulted in the complete loss of the ability to grow on methylamine.

Original languageEnglish
Pages (from-to)217-20
Number of pages4
JournalFEBS Letters
Volume275
Issue number1-2
DOIs
Publication statusPublished - 26 Nov 1990

Keywords

  • Amino Acid Sequence
  • Bacterial Proteins
  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • DNA Mutational Analysis
  • Genes, Bacterial
  • Molecular Sequence Data
  • Oxidoreductases Acting on CH-NH Group Donors
  • Paracoccus denitrificans
  • Regulatory Sequences, Nucleic Acid
  • Restriction Mapping
  • Comparative Study
  • Journal Article
  • Research Support, Non-U.S. Gov't

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