Abstract
The gene encoding the blue-copper protein amicyanin was isolated from a genomic bank of Paracoccus denitrificans by using a synthetic oligonucleotide. It is located directly downstream of the gene encoding the small subunit of methylamine dehydrogenase. Amicyanin is transcribed as a precursor protein with a signal sequence, typical for periplasmic proteins. Specific inactivation of amicyanin by means of gene replacement techniques resulted in the complete loss of the ability to grow on methylamine.
Original language | English |
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Pages (from-to) | 217-20 |
Number of pages | 4 |
Journal | FEBS Letters |
Volume | 275 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - 26 Nov 1990 |
Keywords
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Blotting, Southern
- Cloning, Molecular
- DNA Mutational Analysis
- Genes, Bacterial
- Molecular Sequence Data
- Oxidoreductases Acting on CH-NH Group Donors
- Paracoccus denitrificans
- Regulatory Sequences, Nucleic Acid
- Restriction Mapping
- Comparative Study
- Journal Article
- Research Support, Non-U.S. Gov't