Mutations in ppe38 block PE_PGRS secretion and increase virulence of Mycobacterium tuberculosis

Louis S Ates, Anzaan Dippenaar, Roy Ummels, Sander R Piersma, Aniek D van der Woude, Kim van der Kuij, Fabien Le Chevalier, Dulce Mata-Espinosa, Jorge Barrios-Payán, Brenda Marquina-Castillo, Carolina Guapillo, Connie R Jiménez, Arnab Pain, Edith N G Houben, Robin M Warren, Roland Brosch, Rogelio Hernández-Pando, Wilbert Bitter

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Mycobacterium tuberculosis requires a large number of secreted and exported proteins for its virulence, immune modulation and nutrient uptake. Most of these proteins are transported by the different type VII secretion systems1,2. The most recently evolved type VII secretion system, ESX-5, secretes dozens of substrates belonging to the PE and PPE families, which are named for conserved proline and glutamic acid residues close to the amino terminus3,4. However, the role of these proteins remains largely elusive 1 . Here, we show that mutations of ppe38 completely block the secretion of two large subsets of ESX-5 substrates, that is, PPE-MPTR and PE_PGRS, together comprising >80 proteins. Importantly, hypervirulent clinical M. tuberculosis strains of the Beijing lineage have such a mutation and a concomitant loss of secretion 5 . Restoration of PPE38-dependent secretion partially reverted the hypervirulence phenotype of a Beijing strain, and deletion of ppe38 in moderately virulent M. tuberculosis increased virulence. This indicates that these ESX-5 substrates have an important role in virulence attenuation. Phylogenetic analysis revealed that deletion of ppe38 occurred at the branching point of the 'modern' Beijing sublineage and is shared by Beijing outbreak strains worldwide, suggesting that this deletion may have contributed to their success and global distribution6,7.

Original languageEnglish
Pages (from-to)181-188
Number of pages8
JournalNATURE MICROBIOLOGY
Volume3
Issue number2
DOIs
Publication statusPublished - Feb 2018

Keywords

  • Journal Article

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    Ates, L. S., Dippenaar, A., Ummels, R., Piersma, S. R., van der Woude, A. D., van der Kuij, K., ... Bitter, W. (2018). Mutations in ppe38 block PE_PGRS secretion and increase virulence of Mycobacterium tuberculosis. NATURE MICROBIOLOGY, 3(2), 181-188. https://doi.org/10.1038/s41564-017-0090-6