Mycorrhizal response of Solanum tuberosum to homokaryotic versus dikaryotic arbuscular mycorrhizal fungi

Victoria Terry; Vasilis Kokkoris; Matthew Villeneuve-Laroche; Bianca Turcu; Kendyll Chapman; Calvin Cornell; Zhiming Zheng; Franck Stefani; Nicolas Corradi

doi:10.1007/s00572-023-01123-7

Mycorrhizal response of Solanum tuberosum to homokaryotic versus dikaryotic arbuscular mycorrhizal fungi

Victoria Terry, Vasilis Kokkoris, Matthew Villeneuve-Laroche, Bianca Turcu, Kendyll Chapman, Calvin Cornell, Zhiming Zheng, Franck Stefani, Nicolas Corradi^*

^*Corresponding author for this work

Research output: Contribution to Journal › Article › Academic › peer-review

42 Downloads (Pure)

Abstract

Arbuscular mycorrhizal fungi (AMF) are obligate plant symbionts of most land plants. In these organisms, thousands of nuclei that are either genetically similar (homokaryotic) or derived from two distinct parents (dikaryotic) co-exist in a large syncytium. Here, we investigated the impact of these two nuclear organizations on the mycorrhizal response of potatoes (Solanum tuberosum) by inoculating four potato cultivars with eight Rhizophagus irregularis strains individually (four homokaryotic and four dikaryotic). By evaluating plant and fungal fitness-related traits four months post inoculation, we found that AMF genetic organization significantly affects the mycorrhizal response of host plants. Specifically, homokaryotic strains lead to higher total, shoot, and tuber biomass and a higher number of tubers, compared to dikaryotic strains. However, fungal fitness-related traits showed no clear differences between homokaryotic and dikaryotic strains. Nucleotype content analysis of single spores confirmed that the nucleotype ratio of AMF heterokaryon spores can shift depending on host identity. Together, these findings continue to highlight significant ecological differences derived from the two distinct genetic organizations in AMF.

Original language	English
Pages (from-to)	333-344
Number of pages	12
Journal	Mycorrhiza
Volume	33
Issue number	5-6
Early online date	12 Aug 2023
DOIs	https://doi.org/10.1007/s00572-023-01123-7
Publication status	Published - Nov 2023
Externally published	Yes

Bibliographical note

Funding Information:
Our research was funded by the Discovery Program of the Natural Sciences and Engineering Research Council (RGPIN2020-05643), a Discovery Accelerator Supplements Program (RGPAS-2020–00033). NC is a University of Ottawa Research Chair and VK was supported by the MITACS Industrial PDF program (IT16902) and by the Agriculture and Agri-Food Canada (AAFC) through the project J-002272.

Funding Information:
We thank Andrew Vigars and Savannah Pilgrim for their assistance in lab.

Publisher Copyright:
© 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

Funding

Our research was funded by the Discovery Program of the Natural Sciences and Engineering Research Council (RGPIN2020-05643), a Discovery Accelerator Supplements Program (RGPAS-2020–00033). NC is a University of Ottawa Research Chair and VK was supported by the MITACS Industrial PDF program (IT16902) and by the Agriculture and Agri-Food Canada (AAFC) through the project J-002272. We thank Andrew Vigars and Savannah Pilgrim for their assistance in lab.

Funders	Funder number
Andrew Vigars and Savannah Pilgrim
Discovery Accelerator Supplements Program	RGPAS-2020–00033
Natural Sciences and Engineering Research Council of Canada	RGPIN2020-05643
Natural Sciences and Engineering Research Council of Canada
Agriculture and Agri-Food Canada	J-002272
Agriculture and Agri-Food Canada
Mitacs	IT16902
Mitacs

Keywords

Heterokaryotic
Homokaryotic
Mycorrhizal response (MR)
Nucleotype ratio
Potato
Rhizophagus irregularis

Access to Document

10.1007/s00572-023-01123-7

Mycorrhizal response of Solanum tuberosum to homokaryotic versus dikaryotic arbuscular mycorrhizal fungiFinal published version, 1.25 MBLicence: Article 25fa Dutch Copyright Act

Persistent URL (handle)

Copy link address

Cite this

@article{f9754490b915437fba3a2564fa503a66,

title = "Mycorrhizal response of Solanum tuberosum to homokaryotic versus dikaryotic arbuscular mycorrhizal fungi",

abstract = "Arbuscular mycorrhizal fungi (AMF) are obligate plant symbionts of most land plants. In these organisms, thousands of nuclei that are either genetically similar (homokaryotic) or derived from two distinct parents (dikaryotic) co-exist in a large syncytium. Here, we investigated the impact of these two nuclear organizations on the mycorrhizal response of potatoes (Solanum tuberosum) by inoculating four potato cultivars with eight Rhizophagus irregularis strains individually (four homokaryotic and four dikaryotic). By evaluating plant and fungal fitness-related traits four months post inoculation, we found that AMF genetic organization significantly affects the mycorrhizal response of host plants. Specifically, homokaryotic strains lead to higher total, shoot, and tuber biomass and a higher number of tubers, compared to dikaryotic strains. However, fungal fitness-related traits showed no clear differences between homokaryotic and dikaryotic strains. Nucleotype content analysis of single spores confirmed that the nucleotype ratio of AMF heterokaryon spores can shift depending on host identity. Together, these findings continue to highlight significant ecological differences derived from the two distinct genetic organizations in AMF.",

keywords = "Heterokaryotic, Homokaryotic, Mycorrhizal response (MR), Nucleotype ratio, Potato, Rhizophagus irregularis",

author = "Victoria Terry and Vasilis Kokkoris and Matthew Villeneuve-Laroche and Bianca Turcu and Kendyll Chapman and Calvin Cornell and Zhiming Zheng and Franck Stefani and Nicolas Corradi",

note = "Funding Information: Our research was funded by the Discovery Program of the Natural Sciences and Engineering Research Council (RGPIN2020-05643), a Discovery Accelerator Supplements Program (RGPAS-2020–00033). NC is a University of Ottawa Research Chair and VK was supported by the MITACS Industrial PDF program (IT16902) and by the Agriculture and Agri-Food Canada (AAFC) through the project J-002272. Funding Information: We thank Andrew Vigars and Savannah Pilgrim for their assistance in lab. Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.",

year = "2023",

month = nov,

doi = "10.1007/s00572-023-01123-7",

language = "English",

volume = "33",

pages = "333--344",

journal = "Mycorrhiza",

issn = "0940-6360",

publisher = "Springer Verlag",

number = "5-6",

}

TY - JOUR

T1 - Mycorrhizal response of Solanum tuberosum to homokaryotic versus dikaryotic arbuscular mycorrhizal fungi

AU - Terry, Victoria

AU - Kokkoris, Vasilis

AU - Villeneuve-Laroche, Matthew

AU - Turcu, Bianca

AU - Chapman, Kendyll

AU - Cornell, Calvin

AU - Zheng, Zhiming

AU - Stefani, Franck

AU - Corradi, Nicolas

N1 - Funding Information: Our research was funded by the Discovery Program of the Natural Sciences and Engineering Research Council (RGPIN2020-05643), a Discovery Accelerator Supplements Program (RGPAS-2020–00033). NC is a University of Ottawa Research Chair and VK was supported by the MITACS Industrial PDF program (IT16902) and by the Agriculture and Agri-Food Canada (AAFC) through the project J-002272. Funding Information: We thank Andrew Vigars and Savannah Pilgrim for their assistance in lab. Publisher Copyright: © 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

PY - 2023/11

Y1 - 2023/11

N2 - Arbuscular mycorrhizal fungi (AMF) are obligate plant symbionts of most land plants. In these organisms, thousands of nuclei that are either genetically similar (homokaryotic) or derived from two distinct parents (dikaryotic) co-exist in a large syncytium. Here, we investigated the impact of these two nuclear organizations on the mycorrhizal response of potatoes (Solanum tuberosum) by inoculating four potato cultivars with eight Rhizophagus irregularis strains individually (four homokaryotic and four dikaryotic). By evaluating plant and fungal fitness-related traits four months post inoculation, we found that AMF genetic organization significantly affects the mycorrhizal response of host plants. Specifically, homokaryotic strains lead to higher total, shoot, and tuber biomass and a higher number of tubers, compared to dikaryotic strains. However, fungal fitness-related traits showed no clear differences between homokaryotic and dikaryotic strains. Nucleotype content analysis of single spores confirmed that the nucleotype ratio of AMF heterokaryon spores can shift depending on host identity. Together, these findings continue to highlight significant ecological differences derived from the two distinct genetic organizations in AMF.

AB - Arbuscular mycorrhizal fungi (AMF) are obligate plant symbionts of most land plants. In these organisms, thousands of nuclei that are either genetically similar (homokaryotic) or derived from two distinct parents (dikaryotic) co-exist in a large syncytium. Here, we investigated the impact of these two nuclear organizations on the mycorrhizal response of potatoes (Solanum tuberosum) by inoculating four potato cultivars with eight Rhizophagus irregularis strains individually (four homokaryotic and four dikaryotic). By evaluating plant and fungal fitness-related traits four months post inoculation, we found that AMF genetic organization significantly affects the mycorrhizal response of host plants. Specifically, homokaryotic strains lead to higher total, shoot, and tuber biomass and a higher number of tubers, compared to dikaryotic strains. However, fungal fitness-related traits showed no clear differences between homokaryotic and dikaryotic strains. Nucleotype content analysis of single spores confirmed that the nucleotype ratio of AMF heterokaryon spores can shift depending on host identity. Together, these findings continue to highlight significant ecological differences derived from the two distinct genetic organizations in AMF.

KW - Heterokaryotic

KW - Homokaryotic

KW - Mycorrhizal response (MR)

KW - Nucleotype ratio

KW - Potato

KW - Rhizophagus irregularis

UR - http://www.scopus.com/inward/record.url?scp=85167807350&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85167807350&partnerID=8YFLogxK

U2 - 10.1007/s00572-023-01123-7

DO - 10.1007/s00572-023-01123-7

M3 - Article

C2 - 37572110

SN - 0940-6360

VL - 33

SP - 333

EP - 344

JO - Mycorrhiza

JF - Mycorrhiza

IS - 5-6

ER -

Mycorrhizal response of Solanum tuberosum to homokaryotic versus dikaryotic arbuscular mycorrhizal fungi

Abstract

Bibliographical note

Funding

Keywords

Access to Document

Persistent URL (handle)

Other files and links

Fingerprint

Cite this