NalP-mediated proteolytic release of lactoferrin-binding protein B from the meningococcal cell surface

V. Roussel-Jazede, I. Jongerius, M.P. Bos, J. Tommassen, P. van Ulsen

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Abstract

Bacteria have developed several mechanisms for iron uptake during colonization of mammalian hosts, where the availability of free iron is limiting for growth. Neisseria meningitidis expresses under iron-limiting conditions a receptor complex consisting of the lactoferrin-binding proteins A (LbpA) and LbpB to acquire iron from lactoferrin, which is abundantly present on the mucosal surfaces of the human nasopharynx. LbpA is an integral outer membrane-embedded iron transporter, whereas LbpB is a cell surface-exposed lipoprotein. In this study, we demonstrate that LbpB is also released into the culture medium. We identified NalP, an autotransporter known to be involved in the processing of other autotransporters, as the protease responsible for LbpB release. This release of LbpB reduced the complement-mediated killing of the bacteria when incubated with an LbpB-specific bactericidal antiserum. Since antibodies directed against LbpB are found in convalescent-patient sera, the release of an immunogenic protein as LbpB may represent a novel means for N. meningitidis to escape the human immune response. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
Original languageEnglish
Pages (from-to)3083-3089
Number of pages7
JournalInfection and Immunity
Volume78
Issue number7
DOIs
Publication statusPublished - 2010

Bibliographical note

1098-5522 Roussel-Jazede, Virginie Jongerius, Ilse Bos, Martine P Tommassen, Jan van Ulsen, Peter Journal Article Research Support, Non-U.S. Gov't United States Infect Immun. 2010 Jul;78(7):3083-9. doi: 10.1128/IAI.01193-09. Epub 2010 Apr 26.

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