Nanocrystalline hydroxyapatite-based scaffold adsorbs and gives sustained release of osteoinductive growth factor and facilitates bone regeneration in mice ectopic model

M. Zhou, Y.M. Geng, S.Y. Li, X.B. Yang, Y.J. Che, J.L. Pathak, G. Wu

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Nanocrystalline hydroxyapatite (NHA) is a biocompatible, biodegradable, and osteoconductive bone graft material; however, it lacks osteoinductivity. The present study is aimed at investigating the feasibility of nanocrystalline hydroxyapatite (NHA) as an osteoinductive growth factor carrier. Bone morphogenic protein 2 (BMP2), an osteoinductive growth factor, was incorporated into NHA (BMP2-NHA) using a simple adsorption method. The growth factor loading and release kinetics were profiled using fluorescein-isothiocyanate-labeled bovine serum albumin (FITC-BSA) as a mimic of the osteoinductive growth factor BMP2. The effect of BMP2-NHA on the osteogenic differentiation of C2C12 cells and ectopic bone formation in mice were tested. Confocal laser-scanning microscopy showed that FITC-BSA was diffused throughout the porous structure of NHA. FITC-BSA was efficiently loaded in NHA and sustained release was observed up to 35 days in vitro. BMP2-NHA enhanced the expression of osteogenic markers Runx2, Osterix, Alp, and Col1α1 and ALP activity in C2C12 cells compared to NHA. Similarly, μ-CT and histological examinations showed that BMP2-NHA robustly induced ectopic bone formation in mice. This study suggests that NHA could be used as an effective carrier of osteoinductive growth factors, which ensures osteoinductivity of NHA via sustained release of the growth factor.
Original languageEnglish
Article number1202159
Number of pages10
JournalJournal of Nanomaterials
Volume2019
DOIs
Publication statusPublished - 22 Jan 2019

Bibliographical note

Export Date: 22 October 2019

Funding

The authors thank Shuai shuai Cao and Hui Zhou for the release assay experiment and Professor Hendrick Terhey-den for his guidance during study design. The study was financially supported by the National Key Research and Development Program of China (No. 2016YFC1102900), Guangzhou Science, Technology, and Innovation Commission (No. 201704030024), the Science Foundation for the Youth Scholars of Southern Medical University (No. PY2015N018) and the International Team for Implantology (No. 8812012).

FundersFunder number
Science Foundation for the Youth Scholars of Southern Medical University
Guangzhou Science, Technology and Innovation Commission201704030024
National Basic Research Program of China (973 Program)2016YFC1102900

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