Nanofractionation Spotter Technology for Rapid Contact less and High-Resolution Deposition of LC Eluent for Further Off-Line Analysis

J. Kool, G.E. de Kloe, A.D. Denker, K. van Altena, M.T. Smoluch, D. van Iperen, T.T. Nahar, R.J. Limburg, W.M.A. Niessen, H. Lingeman, R. Leurs, I.J.P. de Esch, A.B. Smit, H. Irth

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

The development of a contactless postcolumn spotter technology capable of rapidly and accurately depositing LC eluent onto another platform (e.g., 1536-well microtiter plates) is described. Many detection methodologies are suitable for online analysis, such as mass spectrometry, UV-vis, and fluorescence. In some cases, when online analysis is less suitable, off-line postcolumn analysis is the methodology of choice and usually relies on LC-based fractionation prior to detection (e.g., MALDI-MS, Raman spectrsocopy, biochemical assays). As fractionation generally involves loss in resolution, the technology described here allows high-resolution contactless fractionation by tailoring the fractionation frequency to the chromatographic peaks and mixing in of postcolumn reagents. Droplet ejection at frequencies of at least 6 Hz could be performed in the nanoliter to low microliter range with repeatabilities of ∼6%. Furthermore, multiple droplets can be ejected at the same position thereby allowing adjustment of fractionation volume and speed. The technology was evaluated, optimized, and validated prior to two proof-of-principle demonstrations comprising off-line chemical detection of injected fluorescein and off-line postcolumn biochemical detection of acetylcholine-binding protein ligands, both based on 1536-well plate reader analysis. © 2010 American Chemical Society.
Original languageEnglish
Pages (from-to)125-132
JournalAnalytical Chemistry
Volume83
Issue number1
DOIs
Publication statusPublished - 2011

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