Nanophotonic measurement of the dark fraction in red fluorescent proteins

J C Prangsma, R. Molenaar, V. Subramaniam, Christian Blum

    Research output: Chapter in Book / Report / Conference proceedingConference contributionAcademicpeer-review

    Abstract

    All complex fluorophores such as fluorescent proteins display a fraction of the population that absorb light but do not emit fluorescence. Accurate measurement of this ‘dark’ fraction is of critical importance for quantitative microscopy in cell biology. We used a nanophotonic method to measure the dark fraction of several frequently used red fluorescent proteins.
    Original languageEnglish
    Title of host publicationProceedings 2015 European Conference on Lasers and Electro-Optics - European Quantum Electronics Conference, CLEO/Europe-EQEC 2015
    PublisherOptical Society of America (OSA)
    ISBN (Electronic)9781467374750
    Publication statusPublished - 2015
    Event2015 European Conference on Lasers and Electro-Optics - European Quantum Electronics Conference, CLEO/Europe-EQEC 2015 - Munich, Germany
    Duration: 21 Jun 201525 Jun 2015

    Conference

    Conference2015 European Conference on Lasers and Electro-Optics - European Quantum Electronics Conference, CLEO/Europe-EQEC 2015
    CountryGermany
    CityMunich
    Period21/06/1525/06/15

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