Nanophotonic measurement of the dark fraction in red fluorescent proteins

J C Prangsma; R. Molenaar; V. Subramaniam; Christian Blum

Nanophotonic measurement of the dark fraction in red fluorescent proteins

J C Prangsma
, R. Molenaar
, V. Subramaniam
, Christian Blum

Research output: Chapter in Book / Report / Conference proceeding › Conference contribution › Academic › peer-review

Abstract

All complex fluorophores such as fluorescent proteins display a fraction of the population that absorb light but do not emit fluorescence. Accurate measurement of this ‘dark’ fraction is of critical importance for quantitative microscopy in cell biology. We used a nanophotonic method to measure the dark fraction of several frequently used red fluorescent proteins.

Original language	English
Title of host publication	Proceedings 2015 European Conference on Lasers and Electro-Optics - European Quantum Electronics Conference, CLEO/Europe-EQEC 2015
Publisher	Optical Society of America (OSA)
ISBN (Electronic)	9781467374750
Publication status	Published - 2015
Event	2015 European Conference on Lasers and Electro-Optics - European Quantum Electronics Conference, CLEO/Europe-EQEC 2015 - Munich, Germany Duration: 21 Jun 2015 → 25 Jun 2015

Conference

Conference	2015 European Conference on Lasers and Electro-Optics - European Quantum Electronics Conference, CLEO/Europe-EQEC 2015
Country/Territory	Germany
City	Munich
Period	21/06/15 → 25/06/15

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https://www.osapublishing.org/abstract.cfm?uri=cleo_europe-2015-CL_2_1

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Prangsma, J. C., Molenaar, R., Subramaniam, V., & Blum, C. (2015). Nanophotonic measurement of the dark fraction in red fluorescent proteins. In Proceedings 2015 European Conference on Lasers and Electro-Optics - European Quantum Electronics Conference, CLEO/Europe-EQEC 2015 Optical Society of America (OSA). https://www.osapublishing.org/abstract.cfm?uri=cleo_europe-2015-CL_2_1

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title = "Nanophotonic measurement of the dark fraction in red fluorescent proteins",

abstract = "All complex fluorophores such as fluorescent proteins display a fraction of the population that absorb light but do not emit fluorescence. Accurate measurement of this {\textquoteleft}dark{\textquoteright} fraction is of critical importance for quantitative microscopy in cell biology. We used a nanophotonic method to measure the dark fraction of several frequently used red fluorescent proteins.",

author = "Prangsma, \{J C\} and R. Molenaar and V. Subramaniam and Christian Blum",

year = "2015",

language = "English",

booktitle = "Proceedings 2015 European Conference on Lasers and Electro-Optics - European Quantum Electronics Conference, CLEO/Europe-EQEC 2015",

publisher = "Optical Society of America (OSA)",

note = "2015 European Conference on Lasers and Electro-Optics - European Quantum Electronics Conference, CLEO/Europe-EQEC 2015 ; Conference date: 21-06-2015 Through 25-06-2015",

}

Prangsma, JC, Molenaar, R, Subramaniam, V & Blum, C 2015, Nanophotonic measurement of the dark fraction in red fluorescent proteins. in Proceedings 2015 European Conference on Lasers and Electro-Optics - European Quantum Electronics Conference, CLEO/Europe-EQEC 2015. Optical Society of America (OSA), 2015 European Conference on Lasers and Electro-Optics - European Quantum Electronics Conference, CLEO/Europe-EQEC 2015, Munich, Germany, 21/06/15. <https://www.osapublishing.org/abstract.cfm?uri=cleo_europe-2015-CL_2_1>

Nanophotonic measurement of the dark fraction in red fluorescent proteins. / Prangsma, J C; Molenaar, R.; Subramaniam, V. et al.
Proceedings 2015 European Conference on Lasers and Electro-Optics - European Quantum Electronics Conference, CLEO/Europe-EQEC 2015. Optical Society of America (OSA), 2015.

Research output: Chapter in Book / Report / Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Nanophotonic measurement of the dark fraction in red fluorescent proteins

AU - Prangsma, J C

AU - Molenaar, R.

AU - Subramaniam, V.

AU - Blum, Christian

PY - 2015

Y1 - 2015

N2 - All complex fluorophores such as fluorescent proteins display a fraction of the population that absorb light but do not emit fluorescence. Accurate measurement of this ‘dark’ fraction is of critical importance for quantitative microscopy in cell biology. We used a nanophotonic method to measure the dark fraction of several frequently used red fluorescent proteins.

AB - All complex fluorophores such as fluorescent proteins display a fraction of the population that absorb light but do not emit fluorescence. Accurate measurement of this ‘dark’ fraction is of critical importance for quantitative microscopy in cell biology. We used a nanophotonic method to measure the dark fraction of several frequently used red fluorescent proteins.

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UR - https://www.scopus.com/pages/publications/85019506041#tab=citedBy

M3 - Conference contribution

AN - SCOPUS:85019506041

BT - Proceedings 2015 European Conference on Lasers and Electro-Optics - European Quantum Electronics Conference, CLEO/Europe-EQEC 2015

PB - Optical Society of America (OSA)

T2 - 2015 European Conference on Lasers and Electro-Optics - European Quantum Electronics Conference, CLEO/Europe-EQEC 2015

Y2 - 21 June 2015 through 25 June 2015

ER -

Nanophotonic measurement of the dark fraction in red fluorescent proteins

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