Neuron-Glia Interactions Increase Neuronal Phenotypes in Tuberous Sclerosis Complex Patient iPSC-Derived Models

Aishwarya G. Nadadhur; Mouhamed Alsaqati; Lisa Gasparotto; Paulien Cornelissen-Steijger; Eline van Hugte; Stephanie Dooves; Adrian J. Harwood; Vivi M. Heine

doi:10.1016/j.stemcr.2018.11.019

Neuron-Glia Interactions Increase Neuronal Phenotypes in Tuberous Sclerosis Complex Patient iPSC-Derived Models

Aishwarya G. Nadadhur, Mouhamed Alsaqati, Lisa Gasparotto, Paulien Cornelissen-Steijger, Eline van Hugte, Stephanie Dooves, Adrian J. Harwood, Vivi M. Heine^*

^*Corresponding author for this work

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Nadadhur et al. generated TSC disease models using patient iPSCs. While neuron mono-cultures showed an increase in network activity and dendritic branching, only when co-cultured with oligodendrocytes (OLs), hypertrophy and axonal abnormalities were observed. Neuron-OL interactions, modulated by mTOR regulators, support use of mixed cultures for TSC disease modeling and drug development.

Original language	English
Pages (from-to)	42-56
Number of pages	15
Journal	Stem cell reports
Volume	12
Issue number	1
DOIs	https://doi.org/10.1016/j.stemcr.2018.11.019
Publication status	Published - 8 Jan 2019

Keywords

autism
co-culture
glia
in vitro model
iPSC
myelin
neuron
neuronal activity
oligodendrocyte
tuberous sclerosis complex

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.stemcr.2018.11.019

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335594

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title = "Neuron-Glia Interactions Increase Neuronal Phenotypes in Tuberous Sclerosis Complex Patient iPSC-Derived Models",

abstract = "Nadadhur et al. generated TSC disease models using patient iPSCs. While neuron mono-cultures showed an increase in network activity and dendritic branching, only when co-cultured with oligodendrocytes (OLs), hypertrophy and axonal abnormalities were observed. Neuron-OL interactions, modulated by mTOR regulators, support use of mixed cultures for TSC disease modeling and drug development.",

keywords = "autism, co-culture, glia, in vitro model, iPSC, myelin, neuron, neuronal activity, oligodendrocyte, tuberous sclerosis complex",

author = "{G. Nadadhur}, Aishwarya and Mouhamed Alsaqati and Lisa Gasparotto and Paulien Cornelissen-Steijger and {van Hugte}, Eline and Stephanie Dooves and Harwood, {Adrian J.} and Heine, {Vivi M.}",

year = "2019",

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doi = "10.1016/j.stemcr.2018.11.019",

language = "English",

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T1 - Neuron-Glia Interactions Increase Neuronal Phenotypes in Tuberous Sclerosis Complex Patient iPSC-Derived Models

AU - G. Nadadhur, Aishwarya

AU - Alsaqati, Mouhamed

AU - Gasparotto, Lisa

AU - Cornelissen-Steijger, Paulien

AU - van Hugte, Eline

AU - Dooves, Stephanie

AU - Harwood, Adrian J.

AU - Heine, Vivi M.

PY - 2019/1/8

Y1 - 2019/1/8

N2 - Nadadhur et al. generated TSC disease models using patient iPSCs. While neuron mono-cultures showed an increase in network activity and dendritic branching, only when co-cultured with oligodendrocytes (OLs), hypertrophy and axonal abnormalities were observed. Neuron-OL interactions, modulated by mTOR regulators, support use of mixed cultures for TSC disease modeling and drug development.

AB - Nadadhur et al. generated TSC disease models using patient iPSCs. While neuron mono-cultures showed an increase in network activity and dendritic branching, only when co-cultured with oligodendrocytes (OLs), hypertrophy and axonal abnormalities were observed. Neuron-OL interactions, modulated by mTOR regulators, support use of mixed cultures for TSC disease modeling and drug development.

KW - autism

KW - co-culture

KW - glia

KW - in vitro model

KW - iPSC

KW - myelin

KW - neuron

KW - neuronal activity

KW - oligodendrocyte

KW - tuberous sclerosis complex

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DO - 10.1016/j.stemcr.2018.11.019

M3 - Article

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VL - 12

SP - 42

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JO - Stem cell reports

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SN - 2213-6711

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ER -

Neuron-Glia Interactions Increase Neuronal Phenotypes in Tuberous Sclerosis Complex Patient iPSC-Derived Models

Abstract

Keywords

UN SDGs

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