Non-invasive in vivo angiography of the human eye with Doppler Optical Coherence Tomography

Boy Braaf, Kari V. Vienola, Koenraad A. Vermeer, Johannes F. de Boer

Research output: Contribution to ConferencePosterOther research output


Optical coherence tomography (OCT) uses laser interferometry for non-invasive cross-sectional imaging of tissues with micrometer resolution. This technology is therefore ideal to visualize the micro-structures of the human retina and choroid in vivo. Additionally blood flow can be detected from Doppler frequency shifts in the OCT signal over time, which are caused by moving particles in flowing blood. In this study we investigated if these Doppler shifts can be used to create angiograms of the retina and choroid.
An experimental OCT system was constructed based on a 1040 nm swept laser source. A healthy volunteer was imaged over a retinal area of 6.0 × 7.9 mm 2 (20º × 26º). Doppler shifts were evaluated by measuring each location twice and were calculated from phase changes within the OCT signals. Angiograms of the vasculature were created by integration of the phase changes over depth.

The retinal angiogram (Fig. 1(A)) shows blood vessels (in white) down to the capillary level and visualizes clearly the avascular zone of the fovea and the entrance and exit of vessels through the optic disc. The choroidal angiogram (Fig. 1(B)) shows a dense network of large vessels below the retina.

Doppler OCT can produce high-resolution angiograms of the retina and choroid.
Original languageEnglish
Number of pages1
Publication statusPublished - 8 Mar 2013
EventVU University Medical Center Science Exchange Day - VU University Medical Center, Amsterdam, Netherlands
Duration: 8 Mar 20138 Mar 2013


ConferenceVU University Medical Center Science Exchange Day
Abbreviated titleVUmc SED 2013


  • optical coherence tomography
  • ophthalmic imaging
  • angiography


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