TY - JOUR
T1 - Noncanonical Amino Acids
T2 - Bringing New-to-Nature Functionalities to Biocatalysis
AU - Brouwer, Bart
AU - Della-Felice, Franco
AU - Illies, Jan Hendrik
AU - Iglesias-Moncayo, Emilia
AU - Roelfes, Gerard
AU - Drienovská, Ivana
N1 - Publisher Copyright:
© 2024 The Authors. Published by American Chemical Society.
PY - 2024/10/9
Y1 - 2024/10/9
N2 - Biocatalysis has become an important component of modern organic chemistry, presenting an efficient and environmentally friendly approach to synthetic transformations. Advances in molecular biology, computational modeling, and protein engineering have unlocked the full potential of enzymes in various industrial applications. However, the inherent limitations of the natural building blocks have sparked a revolutionary shift. In vivo genetic incorporation of noncanonical amino acids exceeds the conventional 20 amino acids, opening new avenues for innovation. This review provides a comprehensive overview of applications of noncanonical amino acids in biocatalysis. We aim to examine the field from multiple perspectives, ranging from their impact on enzymatic reactions to the creation of novel active sites, and subsequent catalysis of new-to-nature reactions. Finally, we discuss the challenges, limitations, and promising opportunities within this dynamic research domain.
AB - Biocatalysis has become an important component of modern organic chemistry, presenting an efficient and environmentally friendly approach to synthetic transformations. Advances in molecular biology, computational modeling, and protein engineering have unlocked the full potential of enzymes in various industrial applications. However, the inherent limitations of the natural building blocks have sparked a revolutionary shift. In vivo genetic incorporation of noncanonical amino acids exceeds the conventional 20 amino acids, opening new avenues for innovation. This review provides a comprehensive overview of applications of noncanonical amino acids in biocatalysis. We aim to examine the field from multiple perspectives, ranging from their impact on enzymatic reactions to the creation of novel active sites, and subsequent catalysis of new-to-nature reactions. Finally, we discuss the challenges, limitations, and promising opportunities within this dynamic research domain.
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U2 - 10.1021/acs.chemrev.4c00136
DO - 10.1021/acs.chemrev.4c00136
M3 - Review article
C2 - 39329413
AN - SCOPUS:85205920955
SN - 0009-2665
VL - 124
SP - 10877
EP - 10923
JO - Chemical Reviews
JF - Chemical Reviews
IS - 19
ER -