Novel artificial metalloenzymes by in vivo incorporation of metal-binding unnatural amino acids

Ivana Drienovská; Ana Rioz-Martínez; Apparao Draksharapu; Gerard Roelfes

doi:10.1039/c4sc01525h

Novel artificial metalloenzymes by in vivo incorporation of metal-binding unnatural amino acids

Ivana Drienovská, Ana Rioz-Martínez, Apparao Draksharapu, Gerard Roelfes^*

^*Corresponding author for this work

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Artificial metalloenzymes have emerged as an attractive new approach to enantioselective catalysis. Herein, we introduce a novel strategy for preparation of artificial metalloenzymes utilizing amber stop codon suppression methodology for the in vivo incorporation of metal-binding unnatural amino acids. The resulting artificial metalloenzymes were applied in catalytic asymmetric Friedel-Crafts alkylation reactions and up to 83% ee for the product was achieved.

Original language	English
Pages (from-to)	770-776
Number of pages	7
Journal	Chemical Science
Volume	6
Issue number	1
DOIs	https://doi.org/10.1039/c4sc01525h
Publication status	Published - 1 Jan 2015
Externally published	Yes

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AU - Drienovská, Ivana

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AU - Draksharapu, Apparao

AU - Roelfes, Gerard

PY - 2015/1/1

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AB - Artificial metalloenzymes have emerged as an attractive new approach to enantioselective catalysis. Herein, we introduce a novel strategy for preparation of artificial metalloenzymes utilizing amber stop codon suppression methodology for the in vivo incorporation of metal-binding unnatural amino acids. The resulting artificial metalloenzymes were applied in catalytic asymmetric Friedel-Crafts alkylation reactions and up to 83% ee for the product was achieved.

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Novel artificial metalloenzymes by in vivo incorporation of metal-binding unnatural amino acids

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