Observation of microtubules with scanning force microscopy in liquid

P.J. de Pablo, I.A.T. Schaap, C. Schmidt

Research output: Contribution to JournalArticleAcademicpeer-review


We present the application of scanning force microscopy using the jumping mode to investigate microtubules adsorbed to glass in air and in liquid. To fix the microtubules the glass surfaces were silanized with aminopropyl-triethoxy-silane. The observed structures ranged from disrupted microtubules in air to intact microtubules in liquid. Intact microtubules show heights between 20 and 24 nm confirming the diameter found in electron microscopy studies. The force applied by the tip was critical for the microtubule height, indicating deformation by the tip. Internal structure, corresponding to protofilaments, was found.
Original languageEnglish
Pages (from-to)143-146
Number of pages4
Issue number2
Publication statusPublished - 10 Jan 2003


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