Observation of microtubules with scanning force microscopy in liquid

P.J. de Pablo; I.A.T. Schaap; C. Schmidt

doi:10.1088/0957-4484/14/2/308

Observation of microtubules with scanning force microscopy in liquid

P.J. de Pablo
, I.A.T. Schaap
, C. Schmidt

Physics of Living Systems

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

We present the application of scanning force microscopy using the jumping mode to investigate microtubules adsorbed to glass in air and in liquid. To fix the microtubules the glass surfaces were silanized with aminopropyl-triethoxy-silane. The observed structures ranged from disrupted microtubules in air to intact microtubules in liquid. Intact microtubules show heights between 20 and 24 nm confirming the diameter found in electron microscopy studies. The force applied by the tip was critical for the microtubule height, indicating deformation by the tip. Internal structure, corresponding to protofilaments, was found.

Original language	English
Pages (from-to)	143-146
Number of pages	4
Journal	Nanotechnology
Volume	14
Issue number	2
DOIs	https://doi.org/10.1088/0957-4484/14/2/308
Publication status	Published - 10 Jan 2003

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abstract = "We present the application of scanning force microscopy using the jumping mode to investigate microtubules adsorbed to glass in air and in liquid. To fix the microtubules the glass surfaces were silanized with aminopropyl-triethoxy-silane. The observed structures ranged from disrupted microtubules in air to intact microtubules in liquid. Intact microtubules show heights between 20 and 24 nm confirming the diameter found in electron microscopy studies. The force applied by the tip was critical for the microtubule height, indicating deformation by the tip. Internal structure, corresponding to protofilaments, was found. ",

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N2 - We present the application of scanning force microscopy using the jumping mode to investigate microtubules adsorbed to glass in air and in liquid. To fix the microtubules the glass surfaces were silanized with aminopropyl-triethoxy-silane. The observed structures ranged from disrupted microtubules in air to intact microtubules in liquid. Intact microtubules show heights between 20 and 24 nm confirming the diameter found in electron microscopy studies. The force applied by the tip was critical for the microtubule height, indicating deformation by the tip. Internal structure, corresponding to protofilaments, was found.

AB - We present the application of scanning force microscopy using the jumping mode to investigate microtubules adsorbed to glass in air and in liquid. To fix the microtubules the glass surfaces were silanized with aminopropyl-triethoxy-silane. The observed structures ranged from disrupted microtubules in air to intact microtubules in liquid. Intact microtubules show heights between 20 and 24 nm confirming the diameter found in electron microscopy studies. The force applied by the tip was critical for the microtubule height, indicating deformation by the tip. Internal structure, corresponding to protofilaments, was found.

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DO - 10.1088/0957-4484/14/2/308

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EP - 146

JO - Nanotechnology

JF - Nanotechnology

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Observation of microtubules with scanning force microscopy in liquid

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