OECD validation study to assess intra- and inter-laboratory reproducibility of the zebrafish embryo toxicity test for acute aquatic toxicity testing

F. Busquet, R. Strecker, J.M. Rawlings, S.E. Belanger, T. Braunbeck, G.J. Carr, P.H. Cenijn, P. Fochtman, A. Gourmelon, N. Hübler, A. Kleensang, M. Knöbel, C. Kussatz, J. Legler, A. Lillicrap, F. Martínez-Jerónimo, C. Polleichtner, H. Rzodeczko, E. Salinas, K.E. Schneider & 7 others S. Scholz, E.J. van den Brandhof, L.T. van der Ven, S. Walter-Rohde, S. Weigt, H. Witters, M. Halder

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

A The OECD validation study of the zebrafish embryo acute toxicity test (ZFET) for acute aquatic toxicity testing evaluated the ZFET reproducibility by testing 20 chemicals at 5 different concentrations in 3 independent runs in at least 3 laboratories. Stock solutions and test concentrations were analytically confirmed for 11 chemicals. Newly fertilised zebrafish eggs (20/concentration and control) were exposed for 96. h to chemicals. Four apical endpoints were recorded daily as indicators of acute lethality: coagulation of the embryo, lack of somite formation, non-detachment of the tail bud from the yolk sac and lack of heartbeat. Results (LC50 values for 48/96. h exposure) show that the ZFET is a robust method with a good intra- and inter-laboratory reproducibility (CV. <. 30%) for most chemicals and laboratories. The reproducibility was lower (CV > 30%) for some very toxic or volatile chemicals, and chemicals tested close to their limit of solubility. The ZFET is now available as OECD Test Guideline 236.Considering the high predictive capacity of the ZFET demonstrated by Belanger et al. (2013) in their retrospective analysis of acute fish toxicity and fish embryo acute toxicity data, the ZFET is ready to be considered for acute fish toxicity for regulatory purposes. © 2014 The Authors.
Original languageEnglish
Pages (from-to)496-511
JournalRegulatory Toxicology and Pharmacology
Volume69
Issue number3
DOIs
Publication statusPublished - 2014

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Acute Toxicity Tests
Validation Studies
Zebrafish
Toxicity
Embryonic Structures
Testing
Fishes
Fish
Somites
Yolk Sac
Organisation for Economic Co-Operation and Development
Zygote
Poisons
Solubility
Tail
Coagulation
Guidelines

Cite this

Busquet, F. ; Strecker, R. ; Rawlings, J.M. ; Belanger, S.E. ; Braunbeck, T. ; Carr, G.J. ; Cenijn, P.H. ; Fochtman, P. ; Gourmelon, A. ; Hübler, N. ; Kleensang, A. ; Knöbel, M. ; Kussatz, C. ; Legler, J. ; Lillicrap, A. ; Martínez-Jerónimo, F. ; Polleichtner, C. ; Rzodeczko, H. ; Salinas, E. ; Schneider, K.E. ; Scholz, S. ; van den Brandhof, E.J. ; van der Ven, L.T. ; Walter-Rohde, S. ; Weigt, S. ; Witters, H. ; Halder, M. / OECD validation study to assess intra- and inter-laboratory reproducibility of the zebrafish embryo toxicity test for acute aquatic toxicity testing. In: Regulatory Toxicology and Pharmacology. 2014 ; Vol. 69, No. 3. pp. 496-511.
@article{3201065b22f947f38b74b972b0b857ae,
title = "OECD validation study to assess intra- and inter-laboratory reproducibility of the zebrafish embryo toxicity test for acute aquatic toxicity testing",
abstract = "A The OECD validation study of the zebrafish embryo acute toxicity test (ZFET) for acute aquatic toxicity testing evaluated the ZFET reproducibility by testing 20 chemicals at 5 different concentrations in 3 independent runs in at least 3 laboratories. Stock solutions and test concentrations were analytically confirmed for 11 chemicals. Newly fertilised zebrafish eggs (20/concentration and control) were exposed for 96. h to chemicals. Four apical endpoints were recorded daily as indicators of acute lethality: coagulation of the embryo, lack of somite formation, non-detachment of the tail bud from the yolk sac and lack of heartbeat. Results (LC50 values for 48/96. h exposure) show that the ZFET is a robust method with a good intra- and inter-laboratory reproducibility (CV. <. 30{\%}) for most chemicals and laboratories. The reproducibility was lower (CV > 30{\%}) for some very toxic or volatile chemicals, and chemicals tested close to their limit of solubility. The ZFET is now available as OECD Test Guideline 236.Considering the high predictive capacity of the ZFET demonstrated by Belanger et al. (2013) in their retrospective analysis of acute fish toxicity and fish embryo acute toxicity data, the ZFET is ready to be considered for acute fish toxicity for regulatory purposes. {\circledC} 2014 The Authors.",
author = "F. Busquet and R. Strecker and J.M. Rawlings and S.E. Belanger and T. Braunbeck and G.J. Carr and P.H. Cenijn and P. Fochtman and A. Gourmelon and N. H{\"u}bler and A. Kleensang and M. Kn{\"o}bel and C. Kussatz and J. Legler and A. Lillicrap and F. Mart{\'i}nez-Jer{\'o}nimo and C. Polleichtner and H. Rzodeczko and E. Salinas and K.E. Schneider and S. Scholz and {van den Brandhof}, E.J. and {van der Ven}, L.T. and S. Walter-Rohde and S. Weigt and H. Witters and M. Halder",
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Busquet, F, Strecker, R, Rawlings, JM, Belanger, SE, Braunbeck, T, Carr, GJ, Cenijn, PH, Fochtman, P, Gourmelon, A, Hübler, N, Kleensang, A, Knöbel, M, Kussatz, C, Legler, J, Lillicrap, A, Martínez-Jerónimo, F, Polleichtner, C, Rzodeczko, H, Salinas, E, Schneider, KE, Scholz, S, van den Brandhof, EJ, van der Ven, LT, Walter-Rohde, S, Weigt, S, Witters, H & Halder, M 2014, 'OECD validation study to assess intra- and inter-laboratory reproducibility of the zebrafish embryo toxicity test for acute aquatic toxicity testing' Regulatory Toxicology and Pharmacology, vol. 69, no. 3, pp. 496-511. https://doi.org/10.1016/j.yrtph.2014.05.018

OECD validation study to assess intra- and inter-laboratory reproducibility of the zebrafish embryo toxicity test for acute aquatic toxicity testing. / Busquet, F.; Strecker, R.; Rawlings, J.M.; Belanger, S.E.; Braunbeck, T.; Carr, G.J.; Cenijn, P.H.; Fochtman, P.; Gourmelon, A.; Hübler, N.; Kleensang, A.; Knöbel, M.; Kussatz, C.; Legler, J.; Lillicrap, A.; Martínez-Jerónimo, F.; Polleichtner, C.; Rzodeczko, H.; Salinas, E.; Schneider, K.E.; Scholz, S.; van den Brandhof, E.J.; van der Ven, L.T.; Walter-Rohde, S.; Weigt, S.; Witters, H.; Halder, M.

In: Regulatory Toxicology and Pharmacology, Vol. 69, No. 3, 2014, p. 496-511.

Research output: Contribution to JournalArticleAcademicpeer-review

TY - JOUR

T1 - OECD validation study to assess intra- and inter-laboratory reproducibility of the zebrafish embryo toxicity test for acute aquatic toxicity testing

AU - Busquet, F.

AU - Strecker, R.

AU - Rawlings, J.M.

AU - Belanger, S.E.

AU - Braunbeck, T.

AU - Carr, G.J.

AU - Cenijn, P.H.

AU - Fochtman, P.

AU - Gourmelon, A.

AU - Hübler, N.

AU - Kleensang, A.

AU - Knöbel, M.

AU - Kussatz, C.

AU - Legler, J.

AU - Lillicrap, A.

AU - Martínez-Jerónimo, F.

AU - Polleichtner, C.

AU - Rzodeczko, H.

AU - Salinas, E.

AU - Schneider, K.E.

AU - Scholz, S.

AU - van den Brandhof, E.J.

AU - van der Ven, L.T.

AU - Walter-Rohde, S.

AU - Weigt, S.

AU - Witters, H.

AU - Halder, M.

PY - 2014

Y1 - 2014

N2 - A The OECD validation study of the zebrafish embryo acute toxicity test (ZFET) for acute aquatic toxicity testing evaluated the ZFET reproducibility by testing 20 chemicals at 5 different concentrations in 3 independent runs in at least 3 laboratories. Stock solutions and test concentrations were analytically confirmed for 11 chemicals. Newly fertilised zebrafish eggs (20/concentration and control) were exposed for 96. h to chemicals. Four apical endpoints were recorded daily as indicators of acute lethality: coagulation of the embryo, lack of somite formation, non-detachment of the tail bud from the yolk sac and lack of heartbeat. Results (LC50 values for 48/96. h exposure) show that the ZFET is a robust method with a good intra- and inter-laboratory reproducibility (CV. <. 30%) for most chemicals and laboratories. The reproducibility was lower (CV > 30%) for some very toxic or volatile chemicals, and chemicals tested close to their limit of solubility. The ZFET is now available as OECD Test Guideline 236.Considering the high predictive capacity of the ZFET demonstrated by Belanger et al. (2013) in their retrospective analysis of acute fish toxicity and fish embryo acute toxicity data, the ZFET is ready to be considered for acute fish toxicity for regulatory purposes. © 2014 The Authors.

AB - A The OECD validation study of the zebrafish embryo acute toxicity test (ZFET) for acute aquatic toxicity testing evaluated the ZFET reproducibility by testing 20 chemicals at 5 different concentrations in 3 independent runs in at least 3 laboratories. Stock solutions and test concentrations were analytically confirmed for 11 chemicals. Newly fertilised zebrafish eggs (20/concentration and control) were exposed for 96. h to chemicals. Four apical endpoints were recorded daily as indicators of acute lethality: coagulation of the embryo, lack of somite formation, non-detachment of the tail bud from the yolk sac and lack of heartbeat. Results (LC50 values for 48/96. h exposure) show that the ZFET is a robust method with a good intra- and inter-laboratory reproducibility (CV. <. 30%) for most chemicals and laboratories. The reproducibility was lower (CV > 30%) for some very toxic or volatile chemicals, and chemicals tested close to their limit of solubility. The ZFET is now available as OECD Test Guideline 236.Considering the high predictive capacity of the ZFET demonstrated by Belanger et al. (2013) in their retrospective analysis of acute fish toxicity and fish embryo acute toxicity data, the ZFET is ready to be considered for acute fish toxicity for regulatory purposes. © 2014 The Authors.

U2 - 10.1016/j.yrtph.2014.05.018

DO - 10.1016/j.yrtph.2014.05.018

M3 - Article

VL - 69

SP - 496

EP - 511

JO - Regulatory Toxicology and Pharmacology

JF - Regulatory Toxicology and Pharmacology

SN - 0273-2300

IS - 3

ER -