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Of problems and opportunities—How to treat and how to not treat crystallographic fragment screening data

  • Manfred S. Weiss*
  • , Jan Wollenhaupt
  • , Galen J. Correy
  • , James S. Fraser
  • , Andreas Heine
  • , Gerhard Klebe
  • , Tobias Krojer
  • , Marjolein Thunissen
  • , Nicholas M. Pearce
  • *Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

In their recent commentary in Protein Science, Jaskolski et al. analyzed three randomly picked diffraction data sets from fragment-screening group depositions from the PDB and, based on that, they claimed that such data are principally problematic. We demonstrate here that if such data are treated properly, none of the proclaimed criticisms persist.

Original languageEnglish
Article numbere4391
Pages (from-to)1-5
Number of pages5
JournalProtein Science
Volume31
Issue number9
Early online date29 Aug 2022
DOIs
Publication statusPublished - Sept 2022

Bibliographical note

Funding Information:
We would like to thank Daren Fearon and Frank von Delft (Diamond Light Source, UK) for providing the map and model files used to generate Figure 1c. We are also grateful for Hans Wienk (NKI Amsterdam, The Netherlands) for critically reading the manuscript and for many textual improvements.

Publisher Copyright:
© 2022 The Authors. Protein Science published by Wiley Periodicals LLC on behalf of The Protein Society.

Funding

We would like to thank Daren Fearon and Frank von Delft (Diamond Light Source, UK) for providing the map and model files used to generate Figure 1c. We are also grateful for Hans Wienk (NKI Amsterdam, The Netherlands) for critically reading the manuscript and for many textual improvements.

Keywords

  • compositional heterogeneity
  • conformational heterogeneity
  • fragment-screening
  • group depositions
  • low-occupancy ligands
  • PanDDA

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