On-line electrochemistry-bioaffinity screening with parallel HR-LC-MS for the generation and characterization of modified p38α kinase inhibitors.

D. Falck, J.S.B. de Vlieger, M. Giera, M. Honing, H. Irth, W.M.A. Niessen, J. Kool

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

In this study, an integrated approach is developed for the formation, identification and biological characterization of electrochemical conversion products of p38α mitogen-activated protein kinase inhibitors. This work demonstrates the hyphenation of an electrochemical reaction cell with a continuous-flow bioaffinity assay and parallel LCHR-MS. Competition of the formed products with a tracer (SKF-86002) that shows fluorescence enhancement in the orthosteric binding site of the p38α kinase is the readout for bioaffinity. Parallel HR-MS
Original languageEnglish
Pages (from-to)367-375
JournalAnalytical and Bioanalytical Chemistry
Volume403
DOIs
Publication statusPublished - 2012

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