On-line SPE-CE for the determination of insulin derivatives in biological fluids

N.F.C. Visser, M. van Harmelen, H. Lingeman, H. Irth

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

An on-line SPE-CE system is described for the determination of insulin derivatives in urine, serum and plasma. By combining techniques based on different separation mechanisms, in this case reversed-phase SPE and CE, a more selective sample clean-up is obtained. The described on-line SPE-CE procedure is able to desalt and clean biological samples, resulting in more repeatable electrophoretic results as well as a good linearity for urine, serum and plasma samples spiked with insulin derivatives, thus proving the elimination of detrimental effects caused by the sample matrix. The on-line SPE-CE system was linear for urine, serum and plasma samples spiked with insulin derivatives between 5 and 80 mg/l. The repeatability in migration time was below 1% relative standard deviation (R.S.D.). The repeatability of the peak was better (<2.4% R.S.D.) when no off-line precipitation reaction (<6.2% R.S.D.) was used, proving the beneficial characteristics of on-line sample pretreatment procedures over off-line sample pretreatment procedures which are prone to sample losses and contamination. © 2003 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)451-62
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume33
Issue number3
DOIs
Publication statusPublished - 2003

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