One-Dimensional STED Microscopy in Optical Tweezers

Tianlong Man; Joost J. Geldhof; Erwin J.G. Peterman; Gijs J.L. Wuite; Iddo Heller

doi:10.1007/978-1-0716-2229-2_6

One-Dimensional STED Microscopy in Optical Tweezers

Tianlong Man, Joost J. Geldhof, Erwin J.G. Peterman, Gijs J.L. Wuite, Iddo Heller^*

^*Corresponding author for this work

Research output: Chapter in Book / Report / Conference proceeding › Chapter › Academic › peer-review

Abstract

Optical tweezers and fluorescence microscopy are powerful methods for investigating the mechanical and structural properties of biomolecules and for studying the dynamics of the biomolecular processes that these molecules are involved in. Here we provide an outline of the concurrent use of optical tweezers and fluorescence microscopy for analyzing biomolecular processes. In particular, we focus on the use of super-resolution microscopy in optical tweezers, which allows visualization of molecules at the higher molecular densities that are typically encountered in living systems. We provide specific details on the alignment procedures of the optical pathways for confocal fluorescence microscopy and 1D-STED microscopy and elaborate on how to diagnose and correct optical aberrations and STED phase plate misalignments.

Original language	English
Title of host publication	Optical Tweezers
Subtitle of host publication	Methods and Protocols
Editors	Arne Gennerich
Publisher	Humana Press Inc
Pages	101-122
Number of pages	22
ISBN (Electronic)	9781071622292
ISBN (Print)	9781071622285
DOIs	https://doi.org/10.1007/978-1-0716-2229-2_6
Publication status	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2478
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

Aberration correction
Alignment
Dual-trap optical tweezers
Focal-intensity distribution
Stimulated emission depletion microscopy (STED)
Super-resolution fluorescence microscopy

Access to Document

10.1007/978-1-0716-2229-2_6

Persistent URL (handle)

Persistent link

Cite this

@inbook{2abc7b0f989c4430b115b1dd9303fec8,

title = "One-Dimensional STED Microscopy in Optical Tweezers",

abstract = "Optical tweezers and fluorescence microscopy are powerful methods for investigating the mechanical and structural properties of biomolecules and for studying the dynamics of the biomolecular processes that these molecules are involved in. Here we provide an outline of the concurrent use of optical tweezers and fluorescence microscopy for analyzing biomolecular processes. In particular, we focus on the use of super-resolution microscopy in optical tweezers, which allows visualization of molecules at the higher molecular densities that are typically encountered in living systems. We provide specific details on the alignment procedures of the optical pathways for confocal fluorescence microscopy and 1D-STED microscopy and elaborate on how to diagnose and correct optical aberrations and STED phase plate misalignments.",

keywords = "Aberration correction, Alignment, Dual-trap optical tweezers, Focal-intensity distribution, Stimulated emission depletion microscopy (STED), Super-resolution fluorescence microscopy",

author = "Tianlong Man and Geldhof, {Joost J.} and Peterman, {Erwin J.G.} and Wuite, {Gijs J.L.} and Iddo Heller",

note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2022",

doi = "10.1007/978-1-0716-2229-2_6",

language = "English",

isbn = "9781071622285",

series = "Methods in Molecular Biology",

publisher = "Humana Press Inc",

pages = "101--122",

editor = "Arne Gennerich",

booktitle = "Optical Tweezers",

}

TY - CHAP

T1 - One-Dimensional STED Microscopy in Optical Tweezers

AU - Man, Tianlong

AU - Geldhof, Joost J.

AU - Peterman, Erwin J.G.

AU - Wuite, Gijs J.L.

AU - Heller, Iddo

PY - 2022

Y1 - 2022

N2 - Optical tweezers and fluorescence microscopy are powerful methods for investigating the mechanical and structural properties of biomolecules and for studying the dynamics of the biomolecular processes that these molecules are involved in. Here we provide an outline of the concurrent use of optical tweezers and fluorescence microscopy for analyzing biomolecular processes. In particular, we focus on the use of super-resolution microscopy in optical tweezers, which allows visualization of molecules at the higher molecular densities that are typically encountered in living systems. We provide specific details on the alignment procedures of the optical pathways for confocal fluorescence microscopy and 1D-STED microscopy and elaborate on how to diagnose and correct optical aberrations and STED phase plate misalignments.

AB - Optical tweezers and fluorescence microscopy are powerful methods for investigating the mechanical and structural properties of biomolecules and for studying the dynamics of the biomolecular processes that these molecules are involved in. Here we provide an outline of the concurrent use of optical tweezers and fluorescence microscopy for analyzing biomolecular processes. In particular, we focus on the use of super-resolution microscopy in optical tweezers, which allows visualization of molecules at the higher molecular densities that are typically encountered in living systems. We provide specific details on the alignment procedures of the optical pathways for confocal fluorescence microscopy and 1D-STED microscopy and elaborate on how to diagnose and correct optical aberrations and STED phase plate misalignments.

KW - Aberration correction

KW - Alignment

KW - Dual-trap optical tweezers

KW - Focal-intensity distribution

KW - Stimulated emission depletion microscopy (STED)

KW - Super-resolution fluorescence microscopy

UR - http://www.scopus.com/inward/record.url?scp=85137158640&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85137158640&partnerID=8YFLogxK

U2 - 10.1007/978-1-0716-2229-2_6

DO - 10.1007/978-1-0716-2229-2_6

M3 - Chapter

C2 - 36063320

AN - SCOPUS:85137158640

SN - 9781071622285

T3 - Methods in Molecular Biology

SP - 101

EP - 122

BT - Optical Tweezers

A2 - Gennerich, Arne

PB - Humana Press Inc

ER -

One-Dimensional STED Microscopy in Optical Tweezers

Abstract

Publication series

Bibliographical note

Keywords

Access to Document

Persistent URL (handle)

Other files and links

Fingerprint

Cite this