Online biochemical detection of glutathione-S-transferase P1-specific inhibitors in complex mixtures.

J. Kool; M. Eggink; H. van Rossum; S.M. van Liempd; D.A. van Elswijk; H. Irth; J.N.M. Commandeur; J.H.N. Meerman; N.P.E. Vermeulen

doi:10.1177/1087057107299527

Online biochemical detection of glutathione-S-transferase P1-specific inhibitors in complex mixtures.

J. Kool, M. Eggink, H. van Rossum, S.M. van Liempd, D.A. van Elswijk, H. Irth, J.N.M. Commandeur, J.H.N. Meerman, N.P.E. Vermeulen

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

A high-resolution screening (HRS) technology is described, which couples 2 parallel enzyme affinity detection (EAD) systems for substrates and inhibitors of rat cytosolic glutathione-S-transferases (cGSTs) and purified human GST P1 to gradient reversed-phase high-performance liquid chromatography (HPLC). The cGSTs and GST P1 EAD systems were optimized and validated first in flow injection analysis (FIA) mode, and optimized values were subsequently used for HPLC mode. The IC

Original language	English
Pages (from-to)	396-405
Journal	Journal of Biomolecular Screening
Volume	12
Issue number	3
DOIs	https://doi.org/10.1177/1087057107299527
Publication status	Published - 2007

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1177/1087057107299527

Handle.net

Persistent link

Cite this

@article{9443d5341b34401287ae0a9c756d47c1,

title = "Online biochemical detection of glutathione-S-transferase P1-specific inhibitors in complex mixtures.",

abstract = "A high-resolution screening (HRS) technology is described, which couples 2 parallel enzyme affinity detection (EAD) systems for substrates and inhibitors of rat cytosolic glutathione-S-transferases (cGSTs) and purified human GST P1 to gradient reversed-phase high-performance liquid chromatography (HPLC). The cGSTs and GST P1 EAD systems were optimized and validated first in flow injection analysis (FIA) mode, and optimized values were subsequently used for HPLC mode. The IC",

author = "J. Kool and M. Eggink and {van Rossum}, H. and {van Liempd}, S.M. and {van Elswijk}, D.A. and H. Irth and J.N.M. Commandeur and J.H.N. Meerman and N.P.E. Vermeulen",

year = "2007",

doi = "10.1177/1087057107299527",

language = "English",

volume = "12",

pages = "396--405",

journal = "Journal of Biomolecular Screening",

issn = "1087-0571",

publisher = "SAGE Publications Inc.",

number = "3",

}

TY - JOUR

T1 - Online biochemical detection of glutathione-S-transferase P1-specific inhibitors in complex mixtures.

AU - Kool, J.

AU - Eggink, M.

AU - van Rossum, H.

AU - van Liempd, S.M.

AU - van Elswijk, D.A.

AU - Irth, H.

AU - Commandeur, J.N.M.

AU - Meerman, J.H.N.

AU - Vermeulen, N.P.E.

PY - 2007

Y1 - 2007

N2 - A high-resolution screening (HRS) technology is described, which couples 2 parallel enzyme affinity detection (EAD) systems for substrates and inhibitors of rat cytosolic glutathione-S-transferases (cGSTs) and purified human GST P1 to gradient reversed-phase high-performance liquid chromatography (HPLC). The cGSTs and GST P1 EAD systems were optimized and validated first in flow injection analysis (FIA) mode, and optimized values were subsequently used for HPLC mode. The IC

AB - A high-resolution screening (HRS) technology is described, which couples 2 parallel enzyme affinity detection (EAD) systems for substrates and inhibitors of rat cytosolic glutathione-S-transferases (cGSTs) and purified human GST P1 to gradient reversed-phase high-performance liquid chromatography (HPLC). The cGSTs and GST P1 EAD systems were optimized and validated first in flow injection analysis (FIA) mode, and optimized values were subsequently used for HPLC mode. The IC

U2 - 10.1177/1087057107299527

DO - 10.1177/1087057107299527

M3 - Article

SN - 1087-0571

VL - 12

SP - 396

EP - 405

JO - Journal of Biomolecular Screening

JF - Journal of Biomolecular Screening

IS - 3

ER -

Online biochemical detection of glutathione-S-transferase P1-specific inhibitors in complex mixtures.

Abstract

UN SDGs

Access to Document

Handle.net

Fingerprint

Cite this