Online biochemical detection of glutathione-S-transferase P1-specific inhibitors in complex mixtures.

J. Kool, M. Eggink, H. van Rossum, S.M. van Liempd, D.A. van Elswijk, H. Irth, J.N.M. Commandeur, J.H.N. Meerman, N.P.E. Vermeulen

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

A high-resolution screening (HRS) technology is described, which couples 2 parallel enzyme affinity detection (EAD) systems for substrates and inhibitors of rat cytosolic glutathione-S-transferases (cGSTs) and purified human GST P1 to gradient reversed-phase high-performance liquid chromatography (HPLC). The cGSTs and GST P1 EAD systems were optimized and validated first in flow injection analysis (FIA) mode, and optimized values were subsequently used for HPLC mode. The IC
Original languageEnglish
Pages (from-to)396-405
JournalJournal of Biomolecular Screening
Volume12
Issue number3
DOIs
Publication statusPublished - 2007

Fingerprint Dive into the research topics of 'Online biochemical detection of glutathione-S-transferase P1-specific inhibitors in complex mixtures.'. Together they form a unique fingerprint.

  • Cite this