Online Collision-Induced Unfolding of Therapeutic Monoclonal Antibody Glyco-Variants through Direct Hyphenation of Cation Exchange Chromatography with Native Ion Mobility–Mass Spectrometry

Guusje van Schaick; Elena Domínguez-Vega; Jérôme Castel; M. Wuhrer; Oscar Hernandez-Alba; Sarah Cianférani

doi:10.1021/acs.analchem.2c03163

Online Collision-Induced Unfolding of Therapeutic Monoclonal Antibody Glyco-Variants through Direct Hyphenation of Cation Exchange Chromatography with Native Ion Mobility–Mass Spectrometry

Guusje van Schaick, Elena Domínguez-Vega, Jérôme Castel, M. Wuhrer, Oscar Hernandez-Alba, Sarah Cianférani

Research output: Contribution to Journal › Article › Academic › peer-review

Original language	English
Pages (from-to)	3932-3939
Journal	Analytical chemistry
Volume	95
Issue number	8
DOIs	https://doi.org/10.1021/acs.analchem.2c03163
Publication status	Published - 28 Feb 2023

Funding

This work was supported by the Netherlands Organization for Scientific Research (NWO) (SATIN project, Grant No. 731.017.202), the CNRS, the University of Strasbourg, the “Agence Nationale de la Recherche” (ConformAbs project, Grant No. ANR-21-CE29-0009-01), and the French Proteomic Infrastructure (ProFI; Grant No. ANR-10-INBS-08-03). The authors would like to thank GIS IBiSA and Région Grand Est for financial support in purchasing a Synapt G2 HDMS instrument. J.C. acknowledges ANRT and NovaliX for funding his Ph.D.

Funders	Funder number
French Proteomic Infrastructure	ANR-10-INBS-08-03
Agence Nationale de la Recherche	ANR-21-CE29-0009-01
Association Nationale de la Recherche et de la Technologie
Nederlandse Organisatie voor Wetenschappelijk Onderzoek	731.017.202
Université de Strasbourg
Centre National de la Recherche Scientifique

Access to Document

10.1021/acs.analchem.2c03163

Persistent URL (handle)

Persistent link

Cite this

Schaick, G. V., Domínguez-Vega, E., Castel, J., Wuhrer, M., Hernandez-Alba, O., & Cianférani, S. (2023). Online Collision-Induced Unfolding of Therapeutic Monoclonal Antibody Glyco-Variants through Direct Hyphenation of Cation Exchange Chromatography with Native Ion Mobility–Mass Spectrometry. Analytical chemistry, 95(8), 3932-3939. https://doi.org/10.1021/acs.analchem.2c03163

@article{3c731530327a4ddd96ff0aa761bf38a2,

title = "Online Collision-Induced Unfolding of Therapeutic Monoclonal Antibody Glyco-Variants through Direct Hyphenation of Cation Exchange Chromatography with Native Ion Mobility–Mass Spectrometry",

author = "Schaick, {Guusje van} and Elena Dom{\'i}nguez-Vega and J{\'e}r{\^o}me Castel and M. Wuhrer and Oscar Hernandez-Alba and Sarah Cianf{\'e}rani",

year = "2023",

month = feb,

day = "28",

doi = "10.1021/acs.analchem.2c03163",

language = "English",

volume = "95",

pages = "3932--3939",

journal = "Analytical chemistry",

issn = "0003-2700",

publisher = "American Chemical Society",

number = "8",

}

Schaick, GV, Domínguez-Vega, E, Castel, J, Wuhrer, M, Hernandez-Alba, O & Cianférani, S 2023, 'Online Collision-Induced Unfolding of Therapeutic Monoclonal Antibody Glyco-Variants through Direct Hyphenation of Cation Exchange Chromatography with Native Ion Mobility–Mass Spectrometry', Analytical chemistry, vol. 95, no. 8, pp. 3932-3939. https://doi.org/10.1021/acs.analchem.2c03163

Online Collision-Induced Unfolding of Therapeutic Monoclonal Antibody Glyco-Variants through Direct Hyphenation of Cation Exchange Chromatography with Native Ion Mobility–Mass Spectrometry. / Schaick, Guusje van; Domínguez-Vega, Elena; Castel, Jérôme et al.
In: Analytical chemistry, Vol. 95, No. 8, 28.02.2023, p. 3932-3939.

Research output: Contribution to Journal › Article › Academic › peer-review

TY - JOUR

T1 - Online Collision-Induced Unfolding of Therapeutic Monoclonal Antibody Glyco-Variants through Direct Hyphenation of Cation Exchange Chromatography with Native Ion Mobility–Mass Spectrometry

AU - Schaick, Guusje van

AU - Domínguez-Vega, Elena

AU - Castel, Jérôme

AU - Wuhrer, M.

AU - Hernandez-Alba, Oscar

AU - Cianférani, Sarah

PY - 2023/2/28

Y1 - 2023/2/28

UR - https://doi.org/10.1021/acs.analchem.2c03163

U2 - 10.1021/acs.analchem.2c03163

DO - 10.1021/acs.analchem.2c03163

M3 - Article

SN - 0003-2700

VL - 95

SP - 3932

EP - 3939

JO - Analytical chemistry

JF - Analytical chemistry

IS - 8

ER -

Online Collision-Induced Unfolding of Therapeutic Monoclonal Antibody Glyco-Variants through Direct Hyphenation of Cation Exchange Chromatography with Native Ion Mobility–Mass Spectrometry

Funding

Access to Document

Persistent URL (handle)

Other files and links

Cite this