Optical trap stiffness in the presence and absence of spherical aberrations

K.C. Vermeulen, G.J.L. Wuite, G.J.M. Stienen, C. Schmidt

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Abstract

Optical traps are commonly constructed with high-numerical-aperture objectives. Oil-immersion objectives suffer from spherical aberrations when used for imaging in aqueous solutions. The effect of spherical aberrations on trapping strength has been modeled by approximation, and only a few experimental results are available in the case of micrometer-sized particles. We present an experimental study of the dependence of lateral and axial optical-trap stiffness on focusing depth for polystyrene and silica beads of 2 μm diameter by using oil- and water-immersion objectives. We demonstrate a strong depth dependence of trap stiffness with the oil-immersion objective, whereas no depth dependence was observed with the water-immersion objective. © 2006 Optical Society of America.
Original languageEnglish
Pages (from-to)1812-1819
Number of pages8
JournalApplied Optics
Volume45
Issue number8
DOIs
Publication statusPublished - 2006

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Optical trap stiffness in the presence and absence of spherical aberrations

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