Optimal lens design and use in laser-scanning microscopy.

A. Negrean, H.D. Mansvelder

Research output: Contribution to JournalArticleAcademicpeer-review


In laser-scanning microscopy often an off-the-shelf achromatic doublet is used as a scan lens which can reduce the available diffraction-limited field-of-view (FOV) by a factor of 3 and introduce chromatic aberrations that are scan angle dependent. Here we present several simple lens designs of superior quality that fully make use of high-NA low-magnification objectives, offering diffraction-limited imaging over a large FOV and wavelength range. We constructed a two-photon laser-scanning microscope with optimized custom lenses which had a near diffraction limit point-spread-function (PSF) with less than 3.6% variation over a 400 μm FOV and less than 0.5 μm lateral color between 750 and 1050 nm. ©2014 Optical Society of America.
Original languageEnglish
Pages (from-to)1588-1609
JournalBiomedical Optics Express
Issue number5
Publication statusPublished - 2014


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