Optimizing the quality of clinical studies on oral microbiome: A practical guide for planning, performing, and reporting

E. Zaura, V.Y. Pappalardo, M.J. Buijs, C.M.C. Volgenant, B.W. Brandt

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

With this review, we aim to increase the quality standards for clinical studies with microbiome as an output parameter. We critically address the existing body of evidence for good quality practices in oral microbiome studies based on 16S rRNA gene amplicon sequencing. First, we discuss the usefulness of microbiome profile analyses. Is a microbiome study actually the best approach for answering the research question? This is followed by addressing the criteria for the most appropriate study design, sample size, and the necessary data (study metadata) that should be collected. Next, we evaluate the available evidence for best practices in sample collection, transport, storage, and DNA isolation. Finally, an overview of possible sequencing options (eg, 16S rRNA gene hypervariable regions, sequencing platforms), processing and data interpretation approaches, as well as requirements for meaningful data storage, sharing, and reporting are provided.

Original languageEnglish
Pages (from-to)210-236
Number of pages27
JournalPeriodontology 2000
Volume85
Issue number1
DOIs
Publication statusPublished - Feb 2021

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