Abstract
We report successful and selective dual protein assembly on patterned bifunctional βCD-Ni(ii)NTA surfaces, using red fluorescent protein variants with hexahistidine-tags and teal fluorescent protein variants conjugated with a peptide containing three adamantyl groups. We show that dual protein patterns can only be assembled, when opposing supramolecular interactions have been optimized and nonspecific interactions have been sufficiently suppressed.
Original language | English |
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Pages (from-to) | 1615-1618 |
Number of pages | 4 |
Journal | Chemical Communications |
Volume | 54 |
Issue number | 13 |
DOIs | |
Publication status | Published - 14 Feb 2018 |
Funding
We thank Dr R. Rurup and K. Leijenhorst-Groener for plasmids (pET15-TealFPv, pRSETB-mRFP, resp.), D. W. ter Brake for synthetic work and St-ERC (259183), NWO-VIDI (723.012.106), DAAD (D/08/46093) and BMM (P2.02 OAcontrol) for funding.
Funders | Funder number |
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BMM | P2.02 OAcontrol |
NWO-VIDI | 723.012.106 |
St-ERC | 259183 |
Deutscher Akademischer Austauschdienst | D/08/46093 |