Orthogonal supramolecular protein assembly on patterned bifunctional surfaces

D. Wasserberg, J. Cabanas-Danés, V. Subramaniam, J. Huskens, P. Jonkheijm*

*Corresponding author for this work

    Research output: Contribution to JournalArticleAcademicpeer-review

    264 Downloads (Pure)


    We report successful and selective dual protein assembly on patterned bifunctional βCD-Ni(ii)NTA surfaces, using red fluorescent protein variants with hexahistidine-tags and teal fluorescent protein variants conjugated with a peptide containing three adamantyl groups. We show that dual protein patterns can only be assembled, when opposing supramolecular interactions have been optimized and nonspecific interactions have been sufficiently suppressed.

    Original languageEnglish
    Pages (from-to)1615-1618
    Number of pages4
    JournalChemical Communications
    Issue number13
    Publication statusPublished - 14 Feb 2018


    We thank Dr R. Rurup and K. Leijenhorst-Groener for plasmids (pET15-TealFPv, pRSETB-mRFP, resp.), D. W. ter Brake for synthetic work and St-ERC (259183), NWO-VIDI (723.012.106), DAAD (D/08/46093) and BMM (P2.02 OAcontrol) for funding.

    FundersFunder number
    BMMP2.02 OAcontrol
    Deutscher Akademischer AustauschdienstD/08/46093


      Dive into the research topics of 'Orthogonal supramolecular protein assembly on patterned bifunctional surfaces'. Together they form a unique fingerprint.

      Cite this