Orthogonal supramolecular protein assembly on patterned bifunctional surfaces

D. Wasserberg, J. Cabanas-Danés, V. Subramaniam, J. Huskens, P. Jonkheijm*

*Corresponding author for this work

    Research output: Contribution to JournalArticleAcademicpeer-review

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    Abstract

    We report successful and selective dual protein assembly on patterned bifunctional βCD-Ni(ii)NTA surfaces, using red fluorescent protein variants with hexahistidine-tags and teal fluorescent protein variants conjugated with a peptide containing three adamantyl groups. We show that dual protein patterns can only be assembled, when opposing supramolecular interactions have been optimized and nonspecific interactions have been sufficiently suppressed.

    Original languageEnglish
    Pages (from-to)1615-1618
    Number of pages4
    JournalChemical Communications
    Volume54
    Issue number13
    DOIs
    Publication statusPublished - 14 Feb 2018

    Funding

    We thank Dr R. Rurup and K. Leijenhorst-Groener for plasmids (pET15-TealFPv, pRSETB-mRFP, resp.), D. W. ter Brake for synthetic work and St-ERC (259183), NWO-VIDI (723.012.106), DAAD (D/08/46093) and BMM (P2.02 OAcontrol) for funding.

    FundersFunder number
    BMMP2.02 OAcontrol
    NWO-VIDI723.012.106
    St-ERC259183
    Deutscher Akademischer AustauschdienstD/08/46093

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