Orthogonal supramolecular protein assembly on patterned bifunctional surfaces

D. Wasserberg; J. Cabanas-Danés; V. Subramaniam; J. Huskens; P. Jonkheijm

doi:10.1039/c7cc09808a

Orthogonal supramolecular protein assembly on patterned bifunctional surfaces

D. Wasserberg
, J. Cabanas-Danés
, V. Subramaniam
, J. Huskens
, P. Jonkheijm^*

^*Corresponding author for this work

Research output: Contribution to Journal › Article › Academic › peer-review

376 Downloads (Pure)

Abstract

We report successful and selective dual protein assembly on patterned bifunctional βCD-Ni(ii)NTA surfaces, using red fluorescent protein variants with hexahistidine-tags and teal fluorescent protein variants conjugated with a peptide containing three adamantyl groups. We show that dual protein patterns can only be assembled, when opposing supramolecular interactions have been optimized and nonspecific interactions have been sufficiently suppressed.

Original language	English
Pages (from-to)	1615-1618
Number of pages	4
Journal	Chemical Communications
Volume	54
Issue number	13
DOIs	https://doi.org/10.1039/c7cc09808a
Publication status	Published - 14 Feb 2018

Funding

We thank Dr R. Rurup and K. Leijenhorst-Groener for plasmids (pET15-TealFPv, pRSETB-mRFP, resp.), D. W. ter Brake for synthetic work and St-ERC (259183), NWO-VIDI (723.012.106), DAAD (D/08/46093) and BMM (P2.02 OAcontrol) for funding.

Funders	Funder number
BMM	P2.02 OAcontrol
NWO-VIDI	723.012.106
St-ERC	259183
Deutscher Akademischer Austauschdienst	D/08/46093

Access to Document

10.1039/c7cc09808a

Orthogonal supramolecular protein assemblyLicence: Article 25fa Dutch Copyright Act

Persistent URL (handle)

Persistent link

Cite this

@article{40e357d5f00c4dc298ae54e279b60162,

title = "Orthogonal supramolecular protein assembly on patterned bifunctional surfaces",

abstract = "We report successful and selective dual protein assembly on patterned bifunctional βCD-Ni(ii)NTA surfaces, using red fluorescent protein variants with hexahistidine-tags and teal fluorescent protein variants conjugated with a peptide containing three adamantyl groups. We show that dual protein patterns can only be assembled, when opposing supramolecular interactions have been optimized and nonspecific interactions have been sufficiently suppressed.",

author = "D. Wasserberg and J. Cabanas-Dan{\'e}s and V. Subramaniam and J. Huskens and P. Jonkheijm",

year = "2018",

month = feb,

day = "14",

doi = "10.1039/c7cc09808a",

language = "English",

volume = "54",

pages = "1615--1618",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "13",

}

TY - JOUR

T1 - Orthogonal supramolecular protein assembly on patterned bifunctional surfaces

AU - Wasserberg, D.

AU - Cabanas-Danés, J.

AU - Subramaniam, V.

AU - Huskens, J.

AU - Jonkheijm, P.

PY - 2018/2/14

Y1 - 2018/2/14

N2 - We report successful and selective dual protein assembly on patterned bifunctional βCD-Ni(ii)NTA surfaces, using red fluorescent protein variants with hexahistidine-tags and teal fluorescent protein variants conjugated with a peptide containing three adamantyl groups. We show that dual protein patterns can only be assembled, when opposing supramolecular interactions have been optimized and nonspecific interactions have been sufficiently suppressed.

AB - We report successful and selective dual protein assembly on patterned bifunctional βCD-Ni(ii)NTA surfaces, using red fluorescent protein variants with hexahistidine-tags and teal fluorescent protein variants conjugated with a peptide containing three adamantyl groups. We show that dual protein patterns can only be assembled, when opposing supramolecular interactions have been optimized and nonspecific interactions have been sufficiently suppressed.

UR - https://www.scopus.com/pages/publications/85041517001

UR - https://www.scopus.com/pages/publications/85041517001#tab=citedBy

U2 - 10.1039/c7cc09808a

DO - 10.1039/c7cc09808a

M3 - Article

AN - SCOPUS:85041517001

SN - 1359-7345

VL - 54

SP - 1615

EP - 1618

JO - Chemical Communications

JF - Chemical Communications

IS - 13

ER -

Orthogonal supramolecular protein assembly on patterned bifunctional surfaces

Abstract

Funding

Access to Document

Persistent URL (handle)

Other files and links

Fingerprint

Cite this