Oxygenic Photoreactivity in Photosystem II Studied by Rotating Ring Disk Electrochemistry

Nikolay Kornienko, Jenny Z. Zhang, Katarzyna P. Sokol, Sarah Lamaison, Andrea Fantuzzi, Rienk Van Grondelle, A. William Rutherford, Erwin Reisner

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Protein film photoelectrochemistry has previously been used to monitor the activity of photosystem II, the water-plastoquinone photooxidoreductase, but the mechanistic information attainable from a three-electrode setup has remained limited. Here we introduce the four-electrode rotating ring disk electrode technique for quantifying light-driven reaction kinetics and mechanistic pathways in real time at the enzyme-electrode interface. This setup allows us to study photochemical H2O oxidation in photosystem II and to gain an in-depth understanding of pathways that generate reactive oxygen species. The results show that photosystem II reacts with O2 through two main pathways that both involve a superoxide intermediate to produce H2O2. The first pathway involves the established chlorophyll triplet-mediated formation of singlet oxygen, which is followed by its reduction to superoxide at the electrode surface. The second pathway is specific for the enzyme/electrode interface: an exposed antenna chlorophyll is sufficiently close to the electrode for rapid injection of an electron to form a highly reducing chlorophyll anion, which reacts with O2 in solution to produce O2 •-. Incomplete H2O oxidation does not significantly contribute to reactive oxygen formation in our conditions. The rotating ring disk electrode technique allows the chemical reactivity of photosystem II to be studied electrochemically and opens several avenues for future investigation.

Original languageEnglish
Pages (from-to)17923-17931
Number of pages9
JournalJournal of the American Chemical Society
Volume140
Issue number51
DOIs
Publication statusPublished - 26 Dec 2018

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Photoreactivity
Electrochemistry
Photosystem II Protein Complex
Electrodes
Chlorophyll
Enzyme electrodes
Biosensing Techniques
Superoxides
Oxygen
Plastoquinone
Oxidation
Chemical reactivity
Singlet Oxygen
Reaction kinetics
Anions
Reactive Oxygen Species
Negative ions
Electrons
Antennas
Proteins

Cite this

Kornienko, Nikolay ; Zhang, Jenny Z. ; Sokol, Katarzyna P. ; Lamaison, Sarah ; Fantuzzi, Andrea ; Van Grondelle, Rienk ; Rutherford, A. William ; Reisner, Erwin. / Oxygenic Photoreactivity in Photosystem II Studied by Rotating Ring Disk Electrochemistry. In: Journal of the American Chemical Society. 2018 ; Vol. 140, No. 51. pp. 17923-17931.
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abstract = "Protein film photoelectrochemistry has previously been used to monitor the activity of photosystem II, the water-plastoquinone photooxidoreductase, but the mechanistic information attainable from a three-electrode setup has remained limited. Here we introduce the four-electrode rotating ring disk electrode technique for quantifying light-driven reaction kinetics and mechanistic pathways in real time at the enzyme-electrode interface. This setup allows us to study photochemical H2O oxidation in photosystem II and to gain an in-depth understanding of pathways that generate reactive oxygen species. The results show that photosystem II reacts with O2 through two main pathways that both involve a superoxide intermediate to produce H2O2. The first pathway involves the established chlorophyll triplet-mediated formation of singlet oxygen, which is followed by its reduction to superoxide at the electrode surface. The second pathway is specific for the enzyme/electrode interface: an exposed antenna chlorophyll is sufficiently close to the electrode for rapid injection of an electron to form a highly reducing chlorophyll anion, which reacts with O2 in solution to produce O2 •-. Incomplete H2O oxidation does not significantly contribute to reactive oxygen formation in our conditions. The rotating ring disk electrode technique allows the chemical reactivity of photosystem II to be studied electrochemically and opens several avenues for future investigation.",
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Kornienko, N, Zhang, JZ, Sokol, KP, Lamaison, S, Fantuzzi, A, Van Grondelle, R, Rutherford, AW & Reisner, E 2018, 'Oxygenic Photoreactivity in Photosystem II Studied by Rotating Ring Disk Electrochemistry' Journal of the American Chemical Society, vol. 140, no. 51, pp. 17923-17931. https://doi.org/10.1021/jacs.8b08784

Oxygenic Photoreactivity in Photosystem II Studied by Rotating Ring Disk Electrochemistry. / Kornienko, Nikolay; Zhang, Jenny Z.; Sokol, Katarzyna P.; Lamaison, Sarah; Fantuzzi, Andrea; Van Grondelle, Rienk; Rutherford, A. William; Reisner, Erwin.

In: Journal of the American Chemical Society, Vol. 140, No. 51, 26.12.2018, p. 17923-17931.

Research output: Contribution to JournalArticleAcademicpeer-review

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