Parallel-plate compression test for soft materials: confocal microscopy-assisted ferrule-top nanoindentation

Dexter Manalili; Massimiliano Berardi; Hilde Aardema; Konstantina Asimaki; Raymund Sarmiento; B. Imran Akca

doi:10.1364/BOE.447147

Parallel-plate compression test for soft materials: confocal microscopy-assisted ferrule-top nanoindentation

Dexter Manalili^*, Massimiliano Berardi, Hilde Aardema, Konstantina Asimaki, Raymund Sarmiento, B. Imran Akca

^*Corresponding author for this work

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

The parallel-plate compression test is one of the simplest ways to measure the mechanical properties of a material. In this test, the Young's modulus (E) and the Poisson's ratio (ν) of the material are determined directly without applying any additional modelling and parameter fitting in the post-processing. This is, however, limited when dealing soft biological materials due to their inherent properties such as being inhomogeneous, microscopic, and overly compliant. By combining an interferometry-assisted parallel-plate compression system and a confocal microscope, we were able to overcome these limitations and measure the E (315 ± 52 Pa) and ν (0.210 ± 0.043) of fixated and permeabilized bovine oocytes.

Original language	English
Pages (from-to)	824-837
Number of pages	14
Journal	Biomedical Optics Express
Volume	13
Issue number	2
Early online date	18 Jan 2022
DOIs	https://doi.org/10.1364/BOE.447147
Publication status	Published - 1 Feb 2022

Bibliographical note

Funding Information:
Acknowledgments. The authors gratefully acknowledge the contributions of Martin Slaman and Erik Paardekam in the fabrication of the ferrule-top indenter and for technical support, Edcel Salumbides for fruitful discussions, and Prof. Davide Iannuzzi for major support since the conceptualization of this work. This work was partly funded by the Science Education Institute, Department of Science and Technology, Republic of the Philippines under the Accelerated Science and Technology Human Resource Development Program’s “Sandwich Program Grant” .

Publisher Copyright:
© 2022 OSA - The Optical Society. All rights reserved.

Funding

Acknowledgments. The authors gratefully acknowledge the contributions of Martin Slaman and Erik Paardekam in the fabrication of the ferrule-top indenter and for technical support, Edcel Salumbides for fruitful discussions, and Prof. Davide Iannuzzi for major support since the conceptualization of this work. This work was partly funded by the Science Education Institute, Department of Science and Technology, Republic of the Philippines under the Accelerated Science and Technology Human Resource Development Program’s “Sandwich Program Grant” .

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title = "Parallel-plate compression test for soft materials: confocal microscopy-assisted ferrule-top nanoindentation",

abstract = "The parallel-plate compression test is one of the simplest ways to measure the mechanical properties of a material. In this test, the Young's modulus (E) and the Poisson's ratio (ν) of the material are determined directly without applying any additional modelling and parameter fitting in the post-processing. This is, however, limited when dealing soft biological materials due to their inherent properties such as being inhomogeneous, microscopic, and overly compliant. By combining an interferometry-assisted parallel-plate compression system and a confocal microscope, we were able to overcome these limitations and measure the E (315 ± 52 Pa) and ν (0.210 ± 0.043) of fixated and permeabilized bovine oocytes.",

author = "Dexter Manalili and Massimiliano Berardi and Hilde Aardema and Konstantina Asimaki and Raymund Sarmiento and Akca, {B. Imran}",

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AU - Sarmiento, Raymund

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Parallel-plate compression test for soft materials: confocal microscopy-assisted ferrule-top nanoindentation

Abstract

Bibliographical note

Funding

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Raw data form oocyte measurements.xlsx

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Parallel-plate compression test for soft materials: confocal microscopy-assisted ferrule-top nanoindentation

Abstract

Bibliographical note

Funding

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Raw data form oocyte measurements.xlsx

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