Parallel scanning laser ophthalmoscope (PSLO) for high-speed retinal imaging

K.V. Vienola, Boy Braaf, Mathi Damodaran, Koenraad A. Vermeer, Johannes F. de Boer

Research output: Contribution to ConferencePosterOther research output

Abstract

Purpose
High-speed imaging of the retina is crucial for obtaining high quality images in the presence of eye motion. To improve the speed of traditional scanners, a high-speed ophthalmic device is presented using a digital micro-mirror device (DMD) for confocal imaging with multiple simultaneous spots.

Methods
The PSLO consists of three parts: an illumination, an imaging and a detector arm (Fig. 1). The DMD is uniformly illuminated with a near-infrared (850 nm) LED. The separation between ON positioned mirror elements was made large enough to eliminate cross-talk between neighboring virtual pinholes, and therefore allowed multi-spot confocal imaging across the whole field of view (FOV). The DMD is programmed to project series of shifted point pattern configurations, effectively scanning the spots over the sample surface. The DMD was imaged onto a sample and the returning light was tapped of via a beam-splitter and imaged on a CMOS camera. Multiple point illuminated frames are combined to form one confocal wide-field image. As a proof of principle images of a resolution target were acquired with the PSLO system.

Results
The resolution target was imaged with a pattern with virtual pinhole size of 2x2 mirrors and the separation between two pinholes was 4 mirror elements. Figure 1B shows the results for combining 9 illumination patterns to form the final image.

Conclusions
It is possible to create wide-field confocal images with the PSLO system. In theory the DMD can achieve higher frame rates than traditional scanner-based systems by illuminating the sample with multiple spots. In retinal imaging, such a setup will provide better images because higher imaging speeds reduce motion artifacts.
Original languageEnglish
Pages1603
Number of pages1
Publication statusPublished - 5 May 2014
EventThe Association for Research in Vision and Ophthalmology Annual Meeting: "Leading Eye and Vision Research" - Orange County Convention Center, Orlando, United States
Duration: 4 May 20148 May 2014
http://www.arvo.org/Conferences_and_Courses/Past_Conferences/

Conference

ConferenceThe Association for Research in Vision and Ophthalmology Annual Meeting
Abbreviated titleARVO 2014
CountryUnited States
CityOrlando
Period4/05/148/05/14
Internet address

Fingerprint

high speed
mirrors
scanning
lasers
pinholes
scanners
illumination
retina
beam splitters
illuminating
field of view
artifacts
CMOS
light emitting diodes
cameras
detectors
configurations

Keywords

  • retinal imaging
  • ophthalmic imaging
  • digital micromirror device
  • structured illumination
  • biomedical optics

Cite this

Vienola, K. V., Braaf, B., Damodaran, M., Vermeer, K. A., & de Boer, J. F. (2014). Parallel scanning laser ophthalmoscope (PSLO) for high-speed retinal imaging. 1603. Poster session presented at The Association for Research in Vision and Ophthalmology Annual Meeting, Orlando, United States.
Vienola, K.V. ; Braaf, Boy ; Damodaran, Mathi ; Vermeer, Koenraad A. ; de Boer, Johannes F. / Parallel scanning laser ophthalmoscope (PSLO) for high-speed retinal imaging. Poster session presented at The Association for Research in Vision and Ophthalmology Annual Meeting, Orlando, United States.1 p.
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Vienola, KV, Braaf, B, Damodaran, M, Vermeer, KA & de Boer, JF 2014, 'Parallel scanning laser ophthalmoscope (PSLO) for high-speed retinal imaging' The Association for Research in Vision and Ophthalmology Annual Meeting, Orlando, United States, 4/05/14 - 8/05/14, pp. 1603.

Parallel scanning laser ophthalmoscope (PSLO) for high-speed retinal imaging. / Vienola, K.V.; Braaf, Boy; Damodaran, Mathi; Vermeer, Koenraad A.; de Boer, Johannes F.

2014. 1603 Poster session presented at The Association for Research in Vision and Ophthalmology Annual Meeting, Orlando, United States.

Research output: Contribution to ConferencePosterOther research output

TY - CONF

T1 - Parallel scanning laser ophthalmoscope (PSLO) for high-speed retinal imaging

AU - Vienola, K.V.

AU - Braaf, Boy

AU - Damodaran, Mathi

AU - Vermeer, Koenraad A.

AU - de Boer, Johannes F.

PY - 2014/5/5

Y1 - 2014/5/5

N2 - PurposeHigh-speed imaging of the retina is crucial for obtaining high quality images in the presence of eye motion. To improve the speed of traditional scanners, a high-speed ophthalmic device is presented using a digital micro-mirror device (DMD) for confocal imaging with multiple simultaneous spots.MethodsThe PSLO consists of three parts: an illumination, an imaging and a detector arm (Fig. 1). The DMD is uniformly illuminated with a near-infrared (850 nm) LED. The separation between ON positioned mirror elements was made large enough to eliminate cross-talk between neighboring virtual pinholes, and therefore allowed multi-spot confocal imaging across the whole field of view (FOV). The DMD is programmed to project series of shifted point pattern configurations, effectively scanning the spots over the sample surface. The DMD was imaged onto a sample and the returning light was tapped of via a beam-splitter and imaged on a CMOS camera. Multiple point illuminated frames are combined to form one confocal wide-field image. As a proof of principle images of a resolution target were acquired with the PSLO system.ResultsThe resolution target was imaged with a pattern with virtual pinhole size of 2x2 mirrors and the separation between two pinholes was 4 mirror elements. Figure 1B shows the results for combining 9 illumination patterns to form the final image.ConclusionsIt is possible to create wide-field confocal images with the PSLO system. In theory the DMD can achieve higher frame rates than traditional scanner-based systems by illuminating the sample with multiple spots. In retinal imaging, such a setup will provide better images because higher imaging speeds reduce motion artifacts.

AB - PurposeHigh-speed imaging of the retina is crucial for obtaining high quality images in the presence of eye motion. To improve the speed of traditional scanners, a high-speed ophthalmic device is presented using a digital micro-mirror device (DMD) for confocal imaging with multiple simultaneous spots.MethodsThe PSLO consists of three parts: an illumination, an imaging and a detector arm (Fig. 1). The DMD is uniformly illuminated with a near-infrared (850 nm) LED. The separation between ON positioned mirror elements was made large enough to eliminate cross-talk between neighboring virtual pinholes, and therefore allowed multi-spot confocal imaging across the whole field of view (FOV). The DMD is programmed to project series of shifted point pattern configurations, effectively scanning the spots over the sample surface. The DMD was imaged onto a sample and the returning light was tapped of via a beam-splitter and imaged on a CMOS camera. Multiple point illuminated frames are combined to form one confocal wide-field image. As a proof of principle images of a resolution target were acquired with the PSLO system.ResultsThe resolution target was imaged with a pattern with virtual pinhole size of 2x2 mirrors and the separation between two pinholes was 4 mirror elements. Figure 1B shows the results for combining 9 illumination patterns to form the final image.ConclusionsIt is possible to create wide-field confocal images with the PSLO system. In theory the DMD can achieve higher frame rates than traditional scanner-based systems by illuminating the sample with multiple spots. In retinal imaging, such a setup will provide better images because higher imaging speeds reduce motion artifacts.

KW - retinal imaging

KW - ophthalmic imaging

KW - digital micromirror device

KW - structured illumination

KW - biomedical optics

M3 - Poster

SP - 1603

ER -

Vienola KV, Braaf B, Damodaran M, Vermeer KA, de Boer JF. Parallel scanning laser ophthalmoscope (PSLO) for high-speed retinal imaging. 2014. Poster session presented at The Association for Research in Vision and Ophthalmology Annual Meeting, Orlando, United States.