Abstract
High-speed imaging of the retina is crucial for obtaining high quality images in the presence of eye motion. To improve the speed of traditional scanners, a high-speed ophthalmic device is presented using a digital micro-mirror device (DMD) for confocal imaging with multiple simultaneous spots.
Methods
The PSLO consists of three parts: an illumination, an imaging and a detector arm (Fig. 1). The DMD is uniformly illuminated with a near-infrared (850 nm) LED. The separation between ON positioned mirror elements was made large enough to eliminate cross-talk between neighboring virtual pinholes, and therefore allowed multi-spot confocal imaging across the whole field of view (FOV). The DMD is programmed to project series of shifted point pattern configurations, effectively scanning the spots over the sample surface. The DMD was imaged onto a sample and the returning light was tapped of via a beam-splitter and imaged on a CMOS camera. Multiple point illuminated frames are combined to form one confocal wide-field image. As a proof of principle images of a resolution target were acquired with the PSLO system.
Results
The resolution target was imaged with a pattern with virtual pinhole size of 2x2 mirrors and the separation between two pinholes was 4 mirror elements. Figure 1B shows the results for combining 9 illumination patterns to form the final image.
Conclusions
It is possible to create wide-field confocal images with the PSLO system. In theory the DMD can achieve higher frame rates than traditional scanner-based systems by illuminating the sample with multiple spots. In retinal imaging, such a setup will provide better images because higher imaging speeds reduce motion artifacts.
| Original language | English |
|---|---|
| Pages | 1603 |
| Number of pages | 1 |
| Publication status | Published - 5 May 2014 |
| Event | The Association for Research in Vision and Ophthalmology Annual Meeting: "Leading Eye and Vision Research" - Orange County Convention Center, Orlando, United States Duration: 4 May 2014 → 8 May 2014 http://www.arvo.org/Conferences_and_Courses/Past_Conferences/ |
Conference
| Conference | The Association for Research in Vision and Ophthalmology Annual Meeting |
|---|---|
| Abbreviated title | ARVO 2014 |
| Country | United States |
| City | Orlando |
| Period | 4/05/14 → 8/05/14 |
| Internet address |
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Keywords
- retinal imaging
- ophthalmic imaging
- digital micromirror device
- structured illumination
- biomedical optics
Cite this
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Parallel scanning laser ophthalmoscope (PSLO) for high-speed retinal imaging. / Vienola, K.V.; Braaf, Boy; Damodaran, Mathi; Vermeer, Koenraad A.; de Boer, Johannes F.
2014. 1603 Poster session presented at The Association for Research in Vision and Ophthalmology Annual Meeting, Orlando, United States.Research output: Contribution to Conference › Poster › Other research output
TY - CONF
T1 - Parallel scanning laser ophthalmoscope (PSLO) for high-speed retinal imaging
AU - Vienola, K.V.
AU - Braaf, Boy
AU - Damodaran, Mathi
AU - Vermeer, Koenraad A.
AU - de Boer, Johannes F.
PY - 2014/5/5
Y1 - 2014/5/5
N2 - PurposeHigh-speed imaging of the retina is crucial for obtaining high quality images in the presence of eye motion. To improve the speed of traditional scanners, a high-speed ophthalmic device is presented using a digital micro-mirror device (DMD) for confocal imaging with multiple simultaneous spots.MethodsThe PSLO consists of three parts: an illumination, an imaging and a detector arm (Fig. 1). The DMD is uniformly illuminated with a near-infrared (850 nm) LED. The separation between ON positioned mirror elements was made large enough to eliminate cross-talk between neighboring virtual pinholes, and therefore allowed multi-spot confocal imaging across the whole field of view (FOV). The DMD is programmed to project series of shifted point pattern configurations, effectively scanning the spots over the sample surface. The DMD was imaged onto a sample and the returning light was tapped of via a beam-splitter and imaged on a CMOS camera. Multiple point illuminated frames are combined to form one confocal wide-field image. As a proof of principle images of a resolution target were acquired with the PSLO system.ResultsThe resolution target was imaged with a pattern with virtual pinhole size of 2x2 mirrors and the separation between two pinholes was 4 mirror elements. Figure 1B shows the results for combining 9 illumination patterns to form the final image.ConclusionsIt is possible to create wide-field confocal images with the PSLO system. In theory the DMD can achieve higher frame rates than traditional scanner-based systems by illuminating the sample with multiple spots. In retinal imaging, such a setup will provide better images because higher imaging speeds reduce motion artifacts.
AB - PurposeHigh-speed imaging of the retina is crucial for obtaining high quality images in the presence of eye motion. To improve the speed of traditional scanners, a high-speed ophthalmic device is presented using a digital micro-mirror device (DMD) for confocal imaging with multiple simultaneous spots.MethodsThe PSLO consists of three parts: an illumination, an imaging and a detector arm (Fig. 1). The DMD is uniformly illuminated with a near-infrared (850 nm) LED. The separation between ON positioned mirror elements was made large enough to eliminate cross-talk between neighboring virtual pinholes, and therefore allowed multi-spot confocal imaging across the whole field of view (FOV). The DMD is programmed to project series of shifted point pattern configurations, effectively scanning the spots over the sample surface. The DMD was imaged onto a sample and the returning light was tapped of via a beam-splitter and imaged on a CMOS camera. Multiple point illuminated frames are combined to form one confocal wide-field image. As a proof of principle images of a resolution target were acquired with the PSLO system.ResultsThe resolution target was imaged with a pattern with virtual pinhole size of 2x2 mirrors and the separation between two pinholes was 4 mirror elements. Figure 1B shows the results for combining 9 illumination patterns to form the final image.ConclusionsIt is possible to create wide-field confocal images with the PSLO system. In theory the DMD can achieve higher frame rates than traditional scanner-based systems by illuminating the sample with multiple spots. In retinal imaging, such a setup will provide better images because higher imaging speeds reduce motion artifacts.
KW - retinal imaging
KW - ophthalmic imaging
KW - digital micromirror device
KW - structured illumination
KW - biomedical optics
M3 - Poster
SP - 1603
ER -